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Characterization of Malignant Peripheral Blood Cells of Juvenile Chronic Myelogenous Leukemia¹

Division of Hematology/Oncology and Allergy, Department of Genetics and Pathology, Research Institute, The Hospital for Sick Children, Toronto, Canada M5G 1X8

Characterization studies were performed on the malignant peripheral blood cells from three patients with juvenile chronic myelogenous leukemia (JCML) by allowing the cells to increase numerically in liquid cultures. JCML cells proliferated rapidly and excessively in the absence of an added humoral growth factor, whereas control peripheral blood cells declined in number when cultured under identical conditions. Clonality of JCML cells was proven by cytogenetic analysis of the proliferating population. JCML cells were exclusively of monocytic lineage as determined by morphology, staining characteristics, and monoclonal antibody identification of cell-specific surface antigens, but cytochemical and functional studies identified aberrant properties indicating defective differentiation. Striking differences from control cells in ultrastructure and topography were also observed by transmission and scanning electron microscopy. These data provide new information on the cellular origin of JCML and form the basis for further study of leukemic cell biology in this disease.

¹ This study was supported by grants from the National Cancer Institute of Canada.

² Fellow of the Terry Fox Foundation of the National Cancer Institute of Canada.

³ To whom requests for reprints should be addressed, at Division of Hematology/Oncology, The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, Canada M5G 1X8.

Received 3/ 5/86. Revised 5/29/86. Revised 7/28/86. Accepted 8/ 5/86.

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