This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schiller, J. H. Articles by Borden, E. C. Search for Related Content PubMed PubMed Citation Articles by Schiller, J. H. Articles by Borden, E. C.

Synergistic Antiproliferative Effects of Human Recombinant 54- or ß_ser-Interferon with -Interferon on Human Cell Lines of Various Histogenesis¹

Departments of Human Oncology, Medicine and Surgery, University of Wisconsin Clinical Cancer Center, Madison 53792, and William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin 53705

The antiproliferative effects of human interferon (IFN), IFN-, IFN-54, and IFN-ß_ser, alone and in combination, were assessed on 10 human cell lines. All IFNs were produced by recombinant technology and purified to homogeneity. In all cell lines except one, the addition of IFN- to either IFN-54 or IFN-ß_ser resulted in a synergistic antiproliferative effect, regardless of individual IFN sensitivities or tissue of origin. The only exception was a normal diploid fibroblast cell in which an additive antiproliferative effect occurred with combination IFN treatment. A malignant fibrosarcoma cell line of similar mesenchymal origin demonstrated a synergistic antiproliferative effect. One cell line was sensitive to both type I and type II IFN alone. Three cell lines were relatively resistant to IFN- but not to IFN-ß_ser, one was relatively resistant to IFN-ß_ser but not to IFN-, and the remainder were resistant to both IFN- and IFN-ß_ser or IFN-54. The addition of IFN-54 to IFN-ß_ser in the SKCO 1 cell line resulted in an antagonistic interaction. Timing experiments with RT112 cells indicate that IFN- and IFN-ß_ser need not be in the media at the same time for the synergistic effect to occur. Combinations of type I and type II IFN thus resulted in synergistic antiproliferative effect for transformed human cells of various histogenesis, including some with a relative resistance to one or both IFN types.

¹ Supported by the Cetus Triton Interferon Program, Grant CA27436 from the National Cancer Institute, the Veterans Administration Research Service, and research funds from the Departments of Surgery and Human Oncology.

² To whom requests for reprints should be addressed.

Received 9/ 6/84. Revised 5/23/85. Revised 10/24/85. Accepted 10/25/85.

This article has been cited by other articles:

				A.-S.A. Ismail, C. J. Van Groeningen, A. Hardcastle, Q.-F. Ren, G. W. Aherne, F. Geoffroy, C. J. Allegra, and J. L. Grem. Modulation of Fluorouracil Cytotoxicity by Interferon-alpha and -gamma Mol. Pharmacol., February 1, 1998; 53(2): 252 - 261. [Abstract] [Full Text]