This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Chaproniere, D. M. Articles by McKeehan, W. L. Search for Related Content PubMed PubMed Citation Articles by Chaproniere, D. M. Articles by McKeehan, W. L.

Serial Culture of Single Adult Human Prostatic Epithelial Cells in Serum-free Medium Containing Low Calcium and a New Growth Factor from Bovine Brain¹

W. Alton Jones Cell Science Center, Inc., Lake Placid, New York 12946

Primary cultures of epithelial cells from human prostate acini proliferate in defined medium. However, the limited availability of human tissue and the lack of knowledge of the conditions required for clonal growth and serial culture of epithelial cells have limited progress in the study of human prostate cell biology. Here we report conditions that permit the proliferation of single epithelial cells from normal, benign hyperplastic, and carcinomatous prostate through three to four serial passages, which represents at least seven to nine cumulative doublings of the cell populations. Primary cultures were prepared from prostatic acini. Monolayers resulting from the outgrowth of epithelial cells from acini were harvested and dissociated into suspensions of single cells which gave rise to discrete colonies in subsequent culture. The requirements for successful serial culture were (a) a low calcium concentration, (b) the presence of a growth factor that is concentrated in bovine neural tissue, (c), detachment of the epithelial cells with collagenase, and (d) harvest of cells before the cell concentration reached 6000 cells/cm² of culture surface. Suspensions of single cells were successfully stored between subcultures in 10% dimethylsulfoxide with 5% fetal bovine serum and revived after storage for up to 2 months in liquid nitrogen.

¹ This study was supported in part by National Institute of Aging Grants AG 03275 and AG 00095, National Cancer Institute Grant CA 37589, and American Cancer Society Grant BC-459.

² To whom requests for reprints should be addressed.

Received 7/ 3/85. Revised 10/15/85. Accepted 10/28/85.

This article has been cited by other articles:

				A. J. Asirvatham, M. Schmidt, B. Gao, and J. Chaudhary Androgens Regulate the Immune/Inflammatory Response and Cell Survival Pathways in Rat Ventral Prostate Epithelial Cells Endocrinology, January 1, 2006; 147(1): 257 - 271. [Abstract] [Full Text] [PDF]

				M. E. Osler, M. S. Chang, and D. M. Bader Bves modulates epithelial integrity through an interaction at the tight junction J. Cell Sci., October 15, 2005; 118(20): 4667 - 4678. [Abstract] [Full Text] [PDF]

				C. Martin, M. Ross, K. E. Chapman, R. Andrew, P. Bollina, J. R. Seckl, and F. K. Habib CYP7B Generates a Selective Estrogen Receptor {beta} Agonist in Human Prostate J. Clin. Endocrinol. Metab., June 1, 2004; 89(6): 2928 - 2935. [Abstract] [Full Text] [PDF]

				S. H. Lang, M. Stark, A. Collins, A. B. Paul, M. J. Stower, and N. J. Maitland Experimental Prostate Epithelial Morphogenesis in Response to Stroma and Three-Dimensional Matrigel Culture Cell Growth Differ., December 1, 2001; 12(12): 631 - 640. [Abstract] [Full Text] [PDF]

				J.-H. Guh, T.-L. Hwang, F.-N. Ko, S.-C. Chueh, M.-K. Lai, and C.-M. Teng Antiproliferative Effect in Human Prostatic Smooth Muscle Cells by Nitric Oxide Donor Mol. Pharmacol., March 1, 1998; 53(3): 467 - 474. [Abstract] [Full Text]

				C. W. Bayne, F. Donnelly, K. Chapman, P. Bollina, C. Buck, and F. K. Habib A Novel Coculture Model for Benign Prostatic Hyperplasia Expressing Both Isoforms of 5{alpha}-Reductase J. Clin. Endocrinol. Metab., January 1, 1998; 83(1): 206 - 213. [Abstract] [Full Text]