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Correlation of Placental Microsomal Activities with Protein Detected by Antibodies to Rabbit Cytochrome P-450 Isozyme 6 in Preparations from Humans Exposed to Polychlorinated Biphenyls, Quaterphenyls, and Dibenzofurans

Biochemical Risk Analysis Branch [T.K.W., T.E.B., and M.W.A.] and Laboratory of Pharmacology [B.A.D., P.E.B., and R.M.P.], National Institute of Environmental Health Sciences, National Institutes of Health, P.O. Box 12233, Research Triangle Park, North Carolina 27709

Placental tissues were obtained from Chinese women in Taiwan who had been exposed to contaminated rice oils containing polychlorinated biphenyls and their thermal degradative products. Exposure via the diet occurred 4–5 years prior to pregnancy. Placental microsomal fractions from eight of the nine exposed subjects studied showed marked elevation of benzo(a)pyrene hydroxylation and 7-ethoxyresorufin O-deethylation activities relative to control subjects. Placental microsomes from exposed subjects were found to contain a protein that cross-reacted with antibodies raised to rabbit cytochrome P-450 isozyme 6, an isozyme induced by polycyclic aromatic hydrocarbons. This protein was not observed with microsomal samples from control subjects. A significant correlation was found between the relative amounts of the immunoreactive protein and benzo(a)-pyrene hydroxylation and 7-ethoxyresorufin O-deethylation activities. The 7-ethoxyresorufin O-deethylation activities were inhibited by -naphthoflavone, a compound known to inhibit activities of rabbit cytochrome P-450, isozyme 6.

¹ To whom requests for reprints should be addressed, at Department of Scientific Affairs, Searle Pharmaceuticals, Inc., Box 5110, Chicago IL 60680.

Received 7/16/85. Revised 10/28/85. Accepted 10/30/85.

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