This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Deen, D. F. Articles by Tofilon, P. J. Search for Related Content PubMed PubMed Citation Articles by Deen, D. F. Articles by Tofilon, P. J.

Prediction of Human Tumor Cell Chemosensitivity Using the Sister Chromatid Exchange Assay¹

Brain Tumor Research Center of the Department of Neurological Surgery [D. F. D., L. E. K., L. J. M., P. J. T.], and the Departments of Radiation Oncology [D. F. D.] and Laboratory Medicine [L. J. M.], School of Medicine, University of California, San Francisco, California 94143

The sister chromatid exchange (SCE) assay has been used to predict the chemosensitivity to 1,3-bis(2-chloroethyl)-1-nitrosourea of various neoplastic cell subpopulations in eight cell lines derived from human brain tumors. Because the SCE assay is based on analysis of individual cells, data obtained can be plotted as frequency histograms of SCEs per chromosome, and the range of chemosensitivities of cell subpopulations can be identified easily. Results suggest that the SCE assay has predictive value as a clinical assay for drugs for which a strong correlation between cell kill and induction of SCEs has been established.

¹ Supported in part by NIH Program Project Grant CA-13525, NIH Grant CA-34351, and the Andres Soriano Cancer Research Fund.

² To whom requests for reprints should be addressed, at Brain Tumor Research Center, 783 HSW, University of California, San Francisco, CA 94143.

³ Present address: Department of Experimental Radiotherapy, M. D. Anderson Hospital and Tumor Institute, Houston, TX 77030.

Received 7/10/85. Revised 12/12/85. Accepted 12/18/85.