This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Sakamoto, H. Articles by Sugimura, T. Search for Related Content PubMed PubMed Citation Articles by Sakamoto, H. Articles by Sugimura, T.

Detection of Mutant Diphtheria Toxin-resistant Chinese Hamster Lung Cells in Situ by Autoradiography¹

National Cancer Center Research Institute, 1-1, Tsukiji 5 chome, Chuo-ku, Tokyo 104, Japan

An autoradiographic method was developed to detect diphtheria toxinresistant (DT^r) Chinese hamster lung cells in situ. With this method, a dose-dependent increase in the number of DT^r cells was observed after exposure of the cells to increasing concentrations of ethyl methanesulfonate (EMS). Segregation of EMS-induced DT^r cells in a sector of single colonies was clearly demonstrated. It was also found by this method that the number of DT^r cells induced by a fixed concentration of EMS was not determined by the number of cell divisions during the expression time but by the period after exposure of the cells to EMS.

¹ This study was partly supported by grants from the Ministry of Health and Welfare and the Ministry of Education, Science and Culture of Japan and by the Princess Takamatsu Cancer Research Fund.

Received 5/28/85. Revised 12/ 3/85. Accepted 12/18/85.