This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Shipley, G. D. Articles by Moses, H. L. Search for Related Content PubMed PubMed Citation Articles by Shipley, G. D. Articles by Moses, H. L.

Reversible Inhibition of Normal Human Prokeratinocyte Proliferation by Type ß Transforming Growth Factor-Growth Inhibitor in Serum-free Medium¹

Departments of Cell Biology [G. D. S., R. J. W., R. E. S., H. L. M.], Dermatology [M. R. P.], and Pathology [R. E. S., H. L. M.], Mayo Clinic/Foundation, Rochester, Minnesota 55905

Type ß transforming growth factor-growth inhibitor (TGFß/GI) causes normal human prokeratinocytes to arrest growth predominantly in the G₁ phase of the cell cycle within 48 h after log phase cultures are exposed to the factor in serum-free medium. The growth arrest induced by TGFß/GI is reversible because the cells from treated cultures can be replated into fresh medium and grown into large colonies. Normal prokeratinocytes are demonstrated to secrete TGFß/GI-like molecules into the culture medium and to have specific cell surface receptors for this molecule. In contrast, a human squamous cell carcinoma, SCC-25, does not arrest growth when exposed to TGFß/GI. These cells, unlike the normal prokeratinocytes, do not exhibit detectable cell surface receptors for the factor.

¹ This work was supported by NIH CA16816 and NIH CA27217 to H. L. M., NIH CA39911 to G. D. S., NIH CA28240 to R. E. S., and the Mayo Foundation.

² To whom requests for reprints should be addressed, at Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37232.

Received 6/18/85. Revised 12/ 3/85. Accepted 1/ 2/86.