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Rescue of a Biologically Active Epstein-Barr Virus from Nonproducer Cells¹

Department of Otorhinolaryngology, School of Medicine [T. T.] and Department of Virology, Cancer Research Institute [H. S., H. O.], Kanazawa University, 13-1 Takara-Marchi, Kanazawa 920, Japan; and Department of Medical Microbiology and Immunology, College of Medicine [T. T., R. G.], and Comprehensive Cancer Center [R. G.], The Ohio State University, Columbus, Ohio 43210

We recently established an epithelial/hybrid cell line, A2L/AH, by fusion of 8-azaguanine-resistant epithelial cells, Ad-AH, with a nonproducer lymphoblastoid cell line, A2L, using lymphocytes derived from the human nasopharynx transformed with the B95-8 strain of Epstein-Barr virus. The treatment of the parental A2L lymphoid cells with iododeoxyuridine led to the expression of Epstein-Barr virus early antigen, but neither virus capsid antigen or induction of Epstein-Barr virus DNA synthesis was observed. However, the treatment of the A2L/AH hybrid cells with iododeoxyuridine led to early antigen, virus capsid antigen and virus DNA synthesis, and the formation of virus particles. The virus rescued from the A2L/AH hybrid cells transforms human cord blood lymphocytes but is not able to induce early antigen in superinfected Raji cells.

¹ This work was supported in part by grant CA-29066 from the National Cancer Institute.

Received 10/23/85. Revised 12/16/85. Accepted 12/17/85.

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