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Expression of Myeloid and Major Histocompatibility Antigens on Small Cell Carcinoma of the Lung Cell Lines Analyzed by Cytofluorography: Modulation by -Interferon¹

Departments of Medicine [E. D. B.], Microbiology [E. D. B.], and Pathology [G. D. S., O. S. P.], Dartmouth Medical School, Hanover, New Hampshire 03756

Recent reports have described the expression of myeloid cell surface antigens on cells of small cell carcinoma of the lung (SCCL). In order to confirm and extend these findings, we have examined by cytofluorography a large panel of well-characterized cell lines derived from SCCL tumors for the expression of several myeloid cell-associated antigens, some of which were not examined in previous reports. In addition, we have studied the expression of Classes I and II HLA antigens on these SCCL cell lines. Finally, we examined the effect of -interferon on the expression of several cell surface markers and on proliferation of SCCL cells. We have found that several SCCL cell lines expressed a M_r 55,000 polypeptide antigen, My23, previously found only on monocytes and monocytic leukemia cells. In addition, the cell lines studied expressed another antigen, defined by monoclonal antibody AML-1-99, which is associated with monocytes and hematopoietic stem cells. We confirmed previous studies that the Leu-7 antigenic determinant is expressed on SCCL cells but observed only minimal or absent binding of monoclonal antibody OKM1 to most cell lines. Class I HLA antigens were present on eight of nine lines examined while Class II HLA was expressed on three of nine lines. -Interferon decreased the proliferative rate of all lines examined. However, this lymphokine was capable of inducing Class I HLA on several lines. The effect of -interferon on other cell surface antigens was variable. These studies confirm that some myeloid cell-associated antigens are expressed on cultured SCCL lines and, additionally, show that their expression can be modulated by immune interferon. Determining the significance of finding myeloid cell-associated antigens on SCCL cells will require further study.

¹ Supported in part by Grants CA31888, CA37868, and CA33853 awarded by the National Cancer Institute, NIH. The Cytofluorograph was the generous gift of the Fannie Rippel Foundation and is partially supported by the Norris Cotton Cancer Center Core Grant CA-23108.

² To whom requests for reprints should be addressed, at Department of Microbiology, Dartmouth Medical School, Hanover, NH 03756.

Received 10/ 2/85. Revised 1/31/86. Accepted 2/ 6/86.