This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Brodt, P. Search for Related Content PubMed PubMed Citation Articles by Brodt, P.

Characterization of Two Highly Metastatic Variants of Lewis Lung Carcinoma with Different Organ Specificities¹

Department of Surgery, Division of Surgical Research, McGill University, Montreal, Quebec H3A 1A4, Canada

The biological properties and metastasis of two sublines of the Lewis lung carcinoma (3LL) which have maintained a stable pattern of organ-selective metastasis have been studied. Subline M-3LL, a lung-specific variant which originated from a lung metastasis of the parent line, metastasized only to the lung following injection of 10⁴–10⁶ tumor cells i.v. or s.c. Lymphatic metastases of this tumor were rarely detected. Subline H-3LL which was developed from a rare, spontaneous hepatic metastasis of the parent line metastasized primarily to the liver, but pulmonary metastases have also been observed. While it grew at local s.c. sites, this tumor metastasized to the regional lymph nodes draining the tumor site, as determined by histology and by bioassay of the lymph nodes following their grafting into new recipient animals. Histologically, the two lines were indistinguishable with the exception of a higher incidence of giant cells detected in tissue sections and culture monolayers of the liver-colonizing variant H-3LL. Ten clones derived from each of the variant lines were expanded in vitro and inoculated i.v. While none of the ten clones derived from line M-3LL gave rise to extrapulmonary metastasis, nine of ten clones derived from Tumor H-3LL gave rise to hepatic metastasis. Highly metastatic clones selected from each tumor were subsequently used to study the patterns of distribution and arrest of radiolabeled tumor cells following their inoculation i.v. No correlation could be found between the initial distribution of the radiolabeled tumor cells and the organ selectivity eventually noted in the site of the metastases.

¹ Supported by grants from the Fonds de la Recherche en Santé du Québec and from the Cancer Research Society of Montreal; presented in part at the 75th and 76th Annual Meetings of the American Association for Cancer Research, May 1984 and May 1985 (1, 2).

² Medical Research Council of Canada Scholar.

Received 8/ 6/85. Revised 12/ 5/85. Accepted 12/12/85.

This article has been cited by other articles:

				S. Li, D. Zhang, L. Yang, J. V. Burnier, N. Wang, R. Lin, E. R. Lee, R. I. Glazer, and P. Brodt The IGF-I Receptor Can Alter the Matrix Metalloproteinase Repertoire of Tumor Cells through Transcriptional Regulation of PKC-{alpha} Mol. Endocrinol., December 1, 2009; 23(12): 2013 - 2025. [Abstract] [Full Text] [PDF]

				N. Wang, T. Thuraisingam, L. Fallavollita, A. Ding, D. Radzioch, and P. Brodt The Secretory Leukocyte Protease Inhibitor Is a Type 1 Insulin-Like Growth Factor Receptor-Regulated Protein that Protects against Liver Metastasis by Attenuating the Host Proinflammatory Response. Cancer Res., March 15, 2006; 66(6): 3062 - 3070. [Abstract] [Full Text] [PDF]

				M. N. Kundranda, M. Henderson, K. J. Carter, L. Gorden, A. Binhazim, S. Ray, T. Baptiste, M. Shokrani, M. L. Leite-Browning, W. Jahnen-Dechent, et al. The Serum Glycoprotein Fetuin-A Promotes Lewis Lung Carcinoma Tumorigenesis via Adhesive-Dependent and Adhesive-Independent Mechanisms Cancer Res., January 15, 2005; 65(2): 499 - 506. [Abstract] [Full Text] [PDF]

				D. Zhang, M. Bar-Eli, S. Meloche, and P. Brodt Dual Regulation of MMP-2 Expression by the Type 1 Insulin-like Growth Factor Receptor: THE PHOSPHATIDYLINOSITOL 3-KINASE/Akt AND Raf/ERK PATHWAYS TRANSMIT OPPOSING SIGNALS J. Biol. Chem., May 7, 2004; 279(19): 19683 - 19690. [Abstract] [Full Text] [PDF]

				Y. Tang, D. Zhang, L. Fallavollita, and P. Brodt Vascular Endothelial Growth Factor C Expression and Lymph Node Metastasis Are Regulated by the Type I Insulin-like Growth Factor Receptor Cancer Res., March 15, 2003; 63(6): 1166 - 1171. [Abstract] [Full Text] [PDF]

				K.-i. Kozaki, O. Miyaishi, T. Tsukamoto, Y. Tatematsu, T. Hida, T. Takahashi, and T. Takahashi Establishment and Characterization of a Human Lung Cancer Cell Line NCI-H460-LNM35 with Consistent Lymphogenous Metastasis via Both Subcutaneous and Orthotopic Propagation Cancer Res., May 1, 2000; 60(9): 2535 - 2540. [Abstract] [Full Text]

				A.-M. Khatib, M. Kontogiannea, L. Fallavollita, B. Jamison, S. Meterissian, and P. Brodt Rapid Induction of Cytokine and E-Selectin Expression in the Liver in Response to Metastatic Tumor Cells Cancer Res., March 1, 1999; 59(6): 1356 - 1361. [Abstract] [Full Text] [PDF]

				P. Brodt, L. Fallavollita, A.-M. Khatib, A. A. Samani, and D. Zhang Cooperative Regulation of the Invasive and Metastatic Phenotypes by Different Domains of the Type I Insulin-like Growth Factor Receptor beta Subunit J. Biol. Chem., August 31, 2001; 276(36): 33608 - 33615. [Abstract] [Full Text] [PDF]