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[Cancer Research 46, 2610-2613, May 1, 1986]
© 1986 American Association for Cancer Research
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Further Characterization of the in Vitro Tumoricidal Activity of Staphylococcal Protein A1

Richard F. Branda2, Wesley J. Miller3 and Ronald D. Soltis

Department of Medicine, University of Vermont, Burlington, Vermont 05405, [R. F. B.], and Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455 [W. J. M., R. D. S.]

Treatment of plasma or serum from leukemic patients with solid phase staphylococcal Protein A induced leukemic blast cell lysis in vitro, but this effect was relatively independent of the amount of immunoglobulin G (IgG) removed. Samples with approximately equal cytotoxic activity contained markedly different IgG levels, while samples with similar IgG levels had a wide range of tumoricidal activity. Assays of plasma samples collected during a perfusion of one plasma volume through a Protein A-Sepharose column indicated that the duration of the procedure had a greater effect on cytotoxic activity than did the amount of IgG removed. Neither added leukemic nor normal IgG significantly improved blast cell viability in treated serum. Cytotoxic activity was not dialyzable and concentrated in the Mr <100,000 fraction of samples separated by filtration. Treated cytotoxic serum samples did not have important C1q binding activity. These results suggest that the in vitro tumoricidal activity of solid phase Protein A is probably due to a toxic substance added to serum during immunoadsorption rather than to its immunoadsorptive capacity.

1 This research was supported by grants from the American Cancer Society (CH-220 and Vermont Division) and grant CA 28234 from the NIH.

2 To whom requests for reprints should be addressed. Recipient of Research Career Development Award CA 00946.

3 Recipient of New Investigator Award CA 32109.

Received 8/ 6/85. Revised 1/ 9/86. Accepted 1/30/86.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Cancer Research Clinical Cancer Research
Cancer Epidemiology Biomarkers & Prevention Molecular Cancer Therapeutics
Molecular Cancer Research Cancer Prevention Research
Cancer Prevention Journals Portal Cancer Reviews Online
Annual Meeting Education Book Meeting Abstracts Online
Copyright © 1986 by the American Association for Cancer Research.