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Quantitative Differences in Host Cell Reactivation of Ultraviolet-damaged Virus in Human Skin Fibroblasts and Epidermal Keratinocytes Cultured from the Same Foreskin Biopsy¹

Swiss Institute for Cancer Research, 1066 Epalinges/Lausanne, Switzerland

Repair efficiency of cultured cells may be estimated by measuring the ability of a particular cell type to support virus damaged by an appropriate agent. In this study we have compared the inactivation of ultraviolet (254 nm)-damaged herpes simplex virus in human fibroblast and epidermal keratinocyte cell lines derived from the same foreskin biopsy and found the epithelial cells to be a factor of 3 times less efficient in supporting the damaged virus. The two different cell types show comparable ultraviolet inactivation of clone-forming ability, indicating that the difference is specific to viral host cell reactivation. This study required the development of a quantitative infectious centers assay for the measurement of viral titer in human epithelial cells, a system which may be of more general application in studies of potential human carcinogens.

¹ This investigation was supported by grants from the Swiss National Science Foundation (3.397.083 and 3.108.085) and the Swiss Cancer League.

Received 9/ 9/85. Revised 12/11/85. Accepted 2/11/86.

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