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Inhibition of IA Positive Reticulum Cell Sarcoma Tumor Cell Growth in Syngeneic SJL/J Mice by Passive Administration of Monoclonal Anti-IA Antibody

Department of Microbiology and Immunology, UCLA School of Medicine, University of California, Los Angeles, California 90024

SJL/J (H-2^s) mice develop spontaneous reticulum cell sarcoma (RCS) tumors at the age of 8–11 months. The RCS tumor expresses IA antigens on the cell surface, stimulates syngeneic T-cell proliferation, and appears to depend on host cells' participation for its own growth. The present study investigates the role of passively administered monoclonal anti-IA antibody on RCS tumor growth. The administration of monoclonal anti-IA^s antibody into SJL/J mice prior to tumor inoculation or at the same time as tumor transplantation resulted in a significant inhibition of tumor growth. Furthermore, pretreatment of RCS tumor with antibody prior to inoculation also resulted in tumor growth inhibition. The inhibition seen in all cases studied was tumor specific, since the use of normal ascites on antibody directed against unrelated antigens resulted in no inhibition of tumor growth. Examination of tumor cells derived from spleen and lymph nodes of antibody treated mice demonstrated that the observed inhibition of tumor growth was the result of a significant depletion of IA positive tumor cells. In contrast to other tumor systems studied to date whereby anti-IA antibody promotes tumor growth, the present findings demonstrate that passive administration of anti-IA antibodies inhibit RCS tumor growth in syngeneic mice. The possible mechanisms involved are discussed.

¹ Supported by USPHS Grants CA 19753 and CA36120 awarded by the National Cancer Institute, Department of Health and Human Services.

Received 8/12/85. Revised 1/14/86. Accepted 3/26/86.