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Measurement of Aflatoxin B₁, Its Metabolites, and DNA Adducts by Synchronous Fluorescence Spectrophotometry

Laboratory of Human Carcinogenesis, Division of Cancer Etiology, National Cancer Institute, Bethesda, Maryland 20892 [C. C. H., G. L., V. L. W., D. M.], and Boston University School of Public Health, Department of Environmental Health, Boston, Massachusetts 02118 [J. G.]

Sensitive and specific methods are needed for measuring human exposure to carcinogens. Synchronous fluorescence spectrophotometry can be used to measure fmol of aflatoxins, their metabolites, and DNA adducts. Computer-assisted analysis of spectra of these agents obtained by synchronous fluorescence spectrophotometry can be displayed as contour maps which are highly specific for each agent. Individual agents in mixtures, e.g. aflatoxins B₁ and M₁, can be identified by fourth derivative spectral analysis. This physical method should complement immunological and other methods to measure aflatoxin B₁, its metabolites, and nucleic acid adducts.

¹ To whom requests for reprints should be addressed, at National Cancer Institute, Building 37, Room 2C07, Bethesda, MD 20892.

Received 7/19/85. Revised 3/12/86. Accepted 4/ 4/86.