This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kikuchi, Y. Articles by Kato, K. Search for Related Content PubMed PubMed Citation Articles by Kikuchi, Y. Articles by Kato, K.

Inhibition of Human Ovarian Cancer Cell Growth in Vitro and in Nude Mice by Prostaglandin D₂¹

Department of Obstetrics and Gynecology, National Defense Medical College, Namiki 3-2, Tokorozawa, Saitama 359, Japan

In vitro and in vivo effects of prostaglandin D₂ on human ovarian tumor growth were examined by using a cell line, designated HR, derived from ascites of a patient with serous cystadenocarcinoma of the ovary. The HR cell proliferation in vitro was dose dependently inhibited between concentrations of 0.1 and 4.0 µg of prostaglandin D₂ per ml. From results of ⁵¹Cr release assays and trypan blue dye exclusion tests the inhibitory effect seemed to result from a direct cytotoxic effect by prostaglandin D₂. All DNA, RNA, and protein syntheses by the HR cells were also inhibited in a dose-dependent manner with exposure time of 48 h to prostaglandin D₂. When 5 x 10⁵ HR cells were inoculated to nude mice, the 50% survival time of them in untreated groups was 52 days after inoculation. Although 4 mg of prostaglandin D₂ per kg caused inhibition of the tumor growth, a significant prolongation of the survival time was not observed. On the other hand, the 50% survival time of nude mice treated with 12 mg of prostaglandin D₂ per kg was significantly (P < 0.05) prolonged to 67 days, in addition to a significant inhibition of the tumor growth.

¹ Supported in part by a grant from the Special Scientific Research Program of the Defense Agency in Japan.

² To whom requests for reprints should be addressed.

Received 11/11/85. Revised 2/20/86. Accepted 4/ 1/86.

This article has been cited by other articles:

				A. G. Banerjee, I. Bhattacharyya, and J. K. Vishwanatha Identification of genes and molecular pathways involved in the progression of premalignant oral epithelia Mol. Cancer Ther., June 1, 2005; 4(6): 865 - 875. [Abstract] [Full Text] [PDF]

				M. Dore, L. C. Cote, A. Mitchell, and J. Sirois Expression of Prostaglandin G/H Synthase Type 1, but not Type 2, in Human Ovarian Adenocarcinomas J. Histochem. Cytochem., January 1, 1998; 46(1): 77 - 84. [Abstract] [Full Text]