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Effects of Epidermal Growth Factor Receptor Concentration on Tumorigenicity of A431 Cells in Nude Mice¹

Department of Medicine, Division of Endocrinology and Metabolism [C. L. M., G. N. G.], Department of Medicine, Division of Hematology and Oncology [C. L. M.], Cancer Biology Program, Cancer Center [M. T. C.], University of California, San Diego, School of Medicine, La Jolla, California 92093; and Memorial Sloan-Kettering Cancer Center, New York, New York 10021 [J. M., H. M.]

To test the relationship between the concentration of epidermal growth factor (EGF) receptors and tumor growth in vivo, we measured the rate of growth of several independently isolated A431 cell lines in athymic mice. This series of A431 clonal variants with differing extents of EGF receptor gene amplification and protein expression were implanted into athymic mice and the time to solid tumor formation and rate of growth were measured. Results of these experiments indicate that the degree of gene amplification and concentration of EGF receptors are directly correlated with the growth of these cells as solid tumors in host animals. Complementary DNA hybridization analysis revealed no change in the extent of gene amplification and expression in implanted cells versus excised tumors nor any evidence of further gene rearrangement in vivo. A high concentration of EGF receptors appears to facilitate the growth of tumor cells in vivo and in vitro.

¹ These studies were supported by research grants from the American Cancer Society; The Council for Tobacco Research-U.S.A.; and NIH grants CA42060 and CA37641 (M. T. C., C. L. M., J. M., H. M.). The work was conducted in part by the Clayton Foundation for Research, California.

² Postdoctoral Fellow supported by NIH Training Grant AM07044 from the National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases.

³ John Mendelsohn Fellow of the University of California, San Diego, Cancer Center.

⁴ Clayton Foundation Investigator.

⁵ To whom requests for reprints should be addressed, at Department of Medicine, M-013E, University of California, San Diego, La Jolla, CA 92093.

Received 3/ 3/86. Accepted 5/ 2/86.

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