This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lipinski, M. Articles by Tursz, T. Search for Related Content PubMed PubMed Citation Articles by Lipinski, M. Articles by Tursz, T.

Neuroectoderm-associated Antigens on Ewing's Sarcoma Cell Lines¹

Laboratoire d'Immunobiologie des Tumeurs, CNRS UA 1156, Institut Gustave Roussy, 94805 Villejuif Cedex, France [M. L., K. B., T. T.]; Centre Léon Bérard, 69373 Lyon Cedex, France [I. P., T. P.]; Biochemical Oncology and Membrane Research, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104 [J. W.]; Centre d'Immunologie de Marseille-Luminy, 13288 Marseille Cedex 9, France [C. G.]; and International Agency for Research on Cancer, 69372s Lyon Cedex, France [G. M. L.]

The histogenesis of Ewing's sarcoma, the second most frequent primary bone tumor in humans, remains controversial. Ten Ewing cell lines were analyzed by immunological methods. Surface antigens recognized on Ewing cells were found to be related to the neuroectoderm lineage. They included ganglioside G_D2, a marker of neuroectodermal tissues and tumors, and an acidic glycolipid detected by monoclonal antibody HNK-1 in the nervous system. The P61 rat monoclonal antibody that reacts with a peptide moiety of neural cell adhesion molecule (N-CAM) and a rabbit antiserum raised to purified mouse N-CAM also stained Ewing cells. Flow cytometry analysis performed using these reagents allowed the definition of four distinct Ewing phenotypes: all reagents equally stained group 1 lines; group 2 lines were strongly reactive with anti-N-CAM reagents, by contrast with a fainter staining with HNK-1 and anti-G_D2 antibodies; all reagents but P61 were strongly reactive with group 3 lines; in group 4, Ewing lines were stained by P61 but only poorly by the anti-N-CAM antiserum. Several antibodies to melanoma and neuroblastoma associated antigens including two monoclonal antibodies to the nerve growth factor receptor were also found to react with Ewing cells. By contrast, all antibodies detecting antigens specifically expressed in hematopoietic cell lineages were totally unreactive. HLA class II antigens were never detected while the level of expression of class I antigens varied to a large extent. Ewing cells are characterized by a specific t(11;22) (q23–24;q12) translocation also observed in neuroepithelioma, a neuroectodermal tumor. Thus, Ewing's sarcoma cells share antigenic and karyotypic features with derivatives of the neuroectoderm possibly indicating a related histogenesis.

¹ Supported in part by Grants 83D7 from the Institut Gustave Roussy and 2098 from the Association pour la Recherche sur le Cancer.

² To whom requests for reprints should be addressed.

Received 6/23/86. Accepted 9/22/86.

This article has been cited by other articles:

				Y. Endo, E. Beauchamp, D. Woods, W. G. Taylor, J. A. Toretsky, A. Uren, and J. S. Rubin Wnt-3a and Dickkopf-1 Stimulate Neurite Outgrowth in Ewing Tumor Cells via a Frizzled3- and c-Jun N-Terminal Kinase-Dependent Mechanism Mol. Cell. Biol., April 1, 2008; 28(7): 2368 - 2379. [Abstract] [Full Text] [PDF]

				Y. Miyagawa, H. Okita, H. Nakaijima, Y. Horiuchi, B. Sato, T. Taguchi, M. Toyoda, Y. U. Katagiri, J. Fujimoto, J.-i. Hata, et al. Inducible Expression of Chimeric EWS/ETS Proteins Confers Ewing's Family Tumor-Like Phenotypes to Human Mesenchymal Progenitor Cells Mol. Cell. Biol., April 1, 2008; 28(7): 2125 - 2137. [Abstract] [Full Text] [PDF]

				F. Baliko, T. Bright, R. Poon, B. Cohen, S. E. Egan, and B. A. Alman Inhibition of Notch Signaling Induces Neural Differentiation in Ewing Sarcoma Am. J. Pathol., May 1, 2007; 170(5): 1686 - 1694. [Abstract] [Full Text] [PDF]

				O. Delattre, J. Zucman, T. Melot, X. S. Garau, J.-M. Zucker, G. M. Lenoir, P. F. Ambros, D. Sheer, C. Turc-Carel, T. J. Triche, et al. The Ewing Family of Tumors -- A Subgroup of Small-Round-Cell Tumors Defined by Specific Chimeric Transcripts N. Engl. J. Med., August 4, 1994; 331(5): 294 - 299. [Abstract] [Full Text]

				J. KIM and J. PELLETIER Molecular genetics of chromosome translocations involving EWS and related family members Physiol Genomics, November 11, 1999; 1(3): 127 - 138. [Abstract] [Full Text] [PDF]