This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reale, F. R. Articles by Bogden, A. Search for Related Content PubMed PubMed Citation Articles by Reale, F. R. Articles by Bogden, A.

Characterization of a Human Malignant Mesothelioma Cell Line (H-MESO-1): A Biphasic Solid and Ascitic Tumor Model¹

Departments of Pathology [F. R. R., J. M. C.], Medicine [T. W. G.], and Pediatrics [P. L. T.], University of Massachusetts Medical School, Worcester, Massachusetts 01605; H. J. D. Orthopedic Institute, New York, New York 10003 [S. G.]; and Bogden Laboratories [A. B.], E G & G Mason Research Institute, Worcester, Massachusetts 01605

Human malignant mesothelioma of the pleura was successfully transplanted s. c. into athymic nude mice and grew as a solid neoplastic mass. Tumor growth resulted in death of the animals between 98 and 161 days after implantation. Minced samples of the growing tumor were propagated as a malignant peritoneal effusion. Animals with malignant ascites died predictably at 32 to 33 days. Light and electron microscopy, and immunocytochemistry demonstrated a similarity of the transplanted solid and fluid malignancies with the human primary mesothelioma. Cytogenetic analysis demonstrated a predominance of cells with a triploid number of identifiable but abnormal human chromosomes. This model, which mimics the clinical behavior of malignant mesothelioma in the human, may be of value in animal trials of chemotherapy and immunotherapy.

¹ Supported in part by Grant R01-CA-39748 from the National Cancer Institute.

² To whom requests for reprints should be addressed, at Department of Pathology, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01605.

Received 5/29/86. Revised 2/11/87. Accepted 3/ 9/87.

This article has been cited by other articles:

				A. Catalano, S. Rodilossi, M. R. Rippo, P. Caprari, and A. Procopio Induction of Stem Cell Factor/c-Kit/Slug Signal Transduction in Multidrug-resistant Malignant Mesothelioma Cells J. Biol. Chem., November 5, 2004; 279(45): 46706 - 46714. [Abstract] [Full Text] [PDF]

				E. W. Gabrielson, M. L. Pinn, J. R. Testa, and F. P. Kuhajda Increased Fatty Acid Synthase is a Therapeutic Target in Mesothelioma Clin. Cancer Res., January 1, 2001; 7(1): 153 - 157. [Abstract] [Full Text]

				C. D. Porter, K. V. Lukacs, G. Box, Y. Takeuchi, and M. K. L. Collins Cationic Liposomes Enhance the Rate of Transduction by a Recombinant Retroviral Vector In Vitro and In Vivo J. Virol., June 1, 1998; 72(6): 4832 - 4840. [Abstract] [Full Text] [PDF]

				V. Raso, M. Brown, and J. McGrath Intracellular Targeting with Low pH-triggered Bispecific Antibodies J. Biol. Chem., October 31, 1997; 272(44): 27623 - 27628. [Abstract] [Full Text] [PDF]