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Use of DNA Image Cytometry in Addition to Flow Cytometry for the Study of Patients with Advanced Ovarian Cancer

Department of Pathology [C. J. R., C. J. C., G. J. F.], Clinical Oncology [C. J. R.], Cytochemistry and Cytometry [J. J. P-Z., W. E. M., J. S. P.], Medical Statistics [J. H.], and Gynecology [P. A. M. H.], Leiden University Hospital, Leiden, The Netherlands

Forty-five patients with advanced ovarian cancer were studied with both DNA flow cytometry (FCM) and automatic DNA image cytometry carried out with the Leiden Television Analysis System (Leytas). There was a significant difference in survival between the diploid and nondiploid cases as determined by FCM. Furthermore, the presence of nuclei with a high DNA content (defined as a DNA content higher than 5C) as determined by Leytas indicated a poor prognosis. When the combined results of FCM and Leytas were taken into account, three different groups of patients could be distinguished. The group of patients with a diploid malignancy (n = 12) had a median survival of more than 60 months. The group of patients (n = 11) with a nondiploid tumor having fewer than 100 nuclei with a high DNA content per 1600 microscope fields formed an intermediate group (median survival, 42 months), whereas the median survival of the remaining patients (n = 22), who had a nondiploid malignancy combined with more than 100 of these nuclei per 1600 microscope fields, was only 15 months. In addition, comparison of the clinical parameters by means of a multivariate analysis (Cox regression model) showed that the combined results of FCM and DNA image cytometry had the largest influence on survival. It is concluded that DNA image cytometry appears to be supplementary to FCM for the study of DNA ploidy abnormalities and that the combined results of these methods have a major influence on the clinical outcome.

¹ Present address: Rotterdam Cancer Institute, Postbox 5201, 3008 AE Rotterdam, The Netherlands.

Received 10/21/86. Revised 4/ 8/87. Accepted 4/14/87.

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