This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sato, M. Articles by Yura, Y. Search for Related Content PubMed PubMed Citation Articles by Sato, M. Articles by Yura, Y.

5-Azacytidine Induction of Stable Myoepithelial and Acinar Cells from a Human Salivary Intercalated Duct Cell Clone¹

Second Department of Oral and Maxillofacial Surgery [M. S., M. A., H. Y., T. Y., Y. Y.] and Department of Laboratory Medicine [Y. H.], Tokushima University School of Dentistry, 3 Kuramoto-cho, Tokushima 770 Japan

A neoplastic human salivary intercalated duct cell clone was cultured in 5 µM 5-azacytidine for 5 days at 37°C; then the cells were trypsinized and subcultured in growth medium without 5-azacytidine. Thereafter, subclones were cloned from the subculture. Of 12 subclones isolated, 7 clonal cell lines were established and characterized. The two subclones composed of cells which were spindle shaped or stellate exhibited phenotypes similar to those of myoepithelial cells such as microfibrils and myosin and formed a myoepithelioma upon transplantation of the cells into nude mice. The other five subclones were composed of polygonal cells with numerous secretory granules in their cytoplasm and containing amylase that seems to be specific to acinar cells; transplantation of these cells into nude mice resulted in production of acinic cell carcinoma. These findings indicate that a neoplastic human salivary intercalated duct cell is capable of at least bidirectional differentiation.

¹ This investigation was supported in part by Grant-in-Aid for Scientific Research 61440083 from the Ministry of Education, Science and Culture of Japan.

² To whom requests for reprints should be addressed.

Received 2/ 6/87. Revised 5/14/87. Accepted 5/21/87.

This article has been cited by other articles:

				A. Sakai, Y. Sugawara, T. Kuroishi, T. Sasano, and S. Sugawara Identification of IL-18 and Th17 Cells in Salivary Glands of Patients with Sjogren's Syndrome, and Amplification of IL-17-Mediated Secretion of Inflammatory Cytokines from Salivary Gland Cells by IL-18 J. Immunol., August 15, 2008; 181(4): 2898 - 2906. [Abstract] [Full Text] [PDF]

				A. Uehara, S. Sugawara, K. Watanabe, S. Echigo, M. Sato, T. Yamaguchi, and H. Takada Constitutive Expression of a Bacterial Pattern Recognition Receptor, CD14, in Human Salivary Glands and Secretion as a Soluble Form in Saliva Clin. Vaccine Immunol., March 1, 2003; 10(2): 286 - 292. [Abstract] [Full Text] [PDF]

				M. Hoffman, M. Kibbey, J. Letterio, and H. Kleinman Role of laminin-1 and TGF-beta 3 in acinar differentiation of a human submandibular gland cell line (HSG) J. Cell Sci., January 8, 1996; 109(8): 2013 - 2021. [Abstract] [PDF]