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An Amplification Unit in Human Melanoma Cells Showing Partial Homology with Sequences of Human Papillomavirus Type 9 and with Nuclear Antigen 1 of the Epstein-Barr Virus¹

Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-6900 Heidelberg, Germany [R. K., A. M., T. K., L. G., H. z. H.]; and I. Medizinische Klinik und Poliklinik der Johannes-Gutenberg Universität Mainz, Langenbeckstr. 1, D-6500 Mainz, Germany [W. D., K-H. M. z. B.]

By partial homology with the DNA of human papillomavirus type 9 a cellular amplification unit was detected which is amplified in melanoma cells but not in Epstein-Barr virus-transformed B cells of two melanoma patients. A 2.4-kilobase EcoRI fragment of this amplification unit was cloned and designated mel/HPV9. At the chromosomal level we detected mel/HPV9 in homogeneously staining regions or in abnormally banded regions containing different marker chromosomes of both melanoma cell lines. DNA sequence analysis of a part of mel/HPV 9 revealed homology with the third internal repeat array of Epstein-Barr virus nuclear antigen 1.

¹ Parts of this work were supported by grants of the Deutsche Forschungsgemeinschaft (DFG Di 245/3-3).

² To whom requests for reprints should be addressed, at I. Medizinische Klinik und Poliklinik der Johannes-Gutenberg Universität Mainz, Langenbeckstr. 1, D-6500 Mainz, Germany.

³ Guest scientist from the Institute of Infectious and Parasitic Diseases, Medical Academy, VI. Zaimov 26, 1504 Sofia, Bulgaria.

Received 12/18/86. Revised 5/14/87. Accepted 5/18/87.