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Reduced Levels of Histones H1° and H1b, and Unaltered Content of Methylated DNA in Rainbow Trout Hepatocellular Carcinoma Chromatin

Department of Biochemistry, Faculty of Medicine, University of Manitoba, 770 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0W3 [J. R. D., G. P. D., B. E. N.], and the Department of Food Science and Technology, Oregon State University, Corvallis, Oregon 97331 [R. M., G. B.]

The levels of histone subtypes and DNA methylation of aflatoxin-induced rainbow trout hepatocellular carcinoma and adult liver nuclei were compared. The hepatocellular carcinoma nuclei were enriched in the ubiquitinated species of histone H2A and depleted in histones H1° and H1b. The 5-methylcytosine content and methylation patterns of the vitellogenin genes and the transcriptionally inactive TPG-3 protamine gene were not altered in the trout hepatocellular carcinoma DNA. Thus, undermethylation of DNA is not a general feature of chemically induced tumors in vivo.

¹ Supported by the National Cancer Institute of Canada.

² Medical Research Council Scholar. To whom requests for reprints should be addressed.

³ Recipient of Manitoba Health Research Council Studentship.

⁴ Supported by grants ES03850, ES00210, and ES00093 from USPHS.

Received 4/23/87. Revised 7/14/87. Accepted 7/24/87.

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