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Presence of Fucolipid Antigens with Mono- and Dimeric X Determinant (Le^x) in the Circulating Immune Complexes of Patients with Adenocarcinoma¹

IMRE Corporation [A. K. S., M. C. S., J. P. B., C. K. G., H. W. S.], Program of Biochemical Oncology/Membrane Research, Fred Hutchinson Cancer Research Center [S-i. H.], Department of Pathobiology, Microbiology, and Immunology, University of Washington [S-i. H.], and Immune Response Program, Pacific Northwest Research Foundation [A. K. S., M. C. S., C. K. G., H. W. S.], Seattle, Washington 98109

A series of fucosylated glycosphingolipids with the Lewis^x (Le^x) determinant (Galß14[Fuc13]GlcNAc) have been shown to accumulate in human adenocarcinomas. Le^x glycolipids were eluted from Protein A-silica columns over which plasma from patients with adenocarcinoma had previously been perfused. The fact that Protein A has strong affinity for IgG and IgG-immune complexes suggested that the Le^x antigens isolated from Protein A eluates were complexed with IgG. Lewis^x antigen eluted from Protein A columns banded in the immune complex-enriched region (below IgG) of neutral sucrose density gradients. A modified Raji cell assay and an anticomplement C1q enzyme-linked immunosorbent assay were also used for measurement of Le^x antigen associated with C3- and C1q-CIC, respectively. Following affinity purification of Le^x-IgG complexes and subsequent dissociation of these immune complexes, human antibodies were isolated which reacted with purified glycosphingolipids containing Le^x. Levels of Le^x-IgG complexes were found to be 2- to 5-fold higher in eluates of Protein A-silica columns perfused with plasma from adenocarcinoma patients compared to eluates from columns perfused with plasma from healthy individuals and patients with other cancers. These assays may prove to be of diagnostic and/or prognostic significance in adenocarcinoma.

¹ Supported in part by SBIR Grant 1R43CA40800-01 from National Institute of Health, and grants from the Cancer Research Institute, Inc., and by IMRE Corporation.

² Scholar of the Leukemia Society of America. To whom requests for reprints should be addressed, at IMRE Corporation, 130 Fifth Avenue North, Seattle, WA 98109.

Received 12/29/86. Revised 6/ 1/87. Accepted 7/30/87.

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