This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Stuehr, D. J. Articles by Marletta, M. A. Search for Related Content PubMed PubMed Citation Articles by Stuehr, D. J. Articles by Marletta, M. A.

Synthesis of Nitrite and Nitrate in Murine Macrophage Cell Lines¹

Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts 02140

Synthesis of nitrite (NO₂^-) and nitrate (NO₃^-) was studied in the macrophage cell lines RAW 264.7, WEHI-3, PU5-1.8, J774A.1, and P388D1 and compared to the synthesis by thioglycolate-elicited peritoneal macrophages from C3H/He and C3H/HeJ mice. Treatment with Escherichia coli lipopolysaccharide (LPS) induced NO₂^-/NO₃^- synthesis by all the cell lines except P388D1, which remained unresponsive at the highest LPS concentration (50 µg/ml). Recombinant murine -interferon induced NO₂^-/NO₃^- synthesis in only two cell lines (PU5-1.8 and RAW 264.7), although it activated synthesis by C3H/He and C3H/HeJ macrophages. Dual signal treatments consisting of lymphokines or -interferon plus LPS stimulated NO₂^-/NO₃^- synthesis by all five cell lines and each line showed enhanced synthesis as compared to that induced by any single stimulus. Heat-killed Bacillus Calmette-Guérin and purified mycobacterial protein derivative stimulated NO₂^-/NO₃^- synthesis in three of five cell lines, while dextran sulfate, zymosan, and the synthetic adjuvant muramyl dipeptide were ineffective. Nitrite represented 50–75% of the total NO₂^-/NO₃^- produced in all cases. The kinetics of LPS-induced NO₂^-/NO₃^- synthesis in J774A.1 and C3H/He macrophages were identical; a 6-h lag phase was followed by a 24- to 48-h period in which NO₂^- and NO₃^- were in a ratio of approximately 3:2 at all time points.

¹ Supported by USPHS Grant CA26731 awarded by the Department of Health and Human Services, National Cancer Institute.

² Present address: Department of Medicine, Cornell University Medical Center, New York, NY 10021.

³ To whom requests for reprints should be addressed, at The University of Michigan, College of Pharmacy, 428 Church St., Ann Arbor, MI 48109.

Received 4/ 6/87. Revised 7/17/87. Accepted 7/24/87.

This article has been cited by other articles:

				I. V. Ustyugova, L. L. Frost, K. VanDyke, K. M. Brundage, R. Schafer, and J. B. Barnett 3,4-Dichloropropionaniline Suppresses Normal Macrophage Function Toxicol. Sci., June 1, 2007; 97(2): 364 - 374. [Abstract] [Full Text] [PDF]

				K. Panda, M. Chawla-Sarkar, C. Santos, T. Koeck, S. C. Erzurum, J. F. Parkinson, and D. J. Stuehr Visualizing inducible nitric-oxide synthase in living cells with a heme-binding fluorescent inhibitor PNAS, July 19, 2005; 102(29): 10117 - 10122. [Abstract] [Full Text] [PDF]

				B. H. Miller, R. A. Fratti, J. F. Poschet, G. S. Timmins, S. S. Master, M. Burgos, M. A. Marletta, and V. Deretic Mycobacteria Inhibit Nitric Oxide Synthase Recruitment to Phagosomes during Macrophage Infection Infect. Immun., May 1, 2004; 72(5): 2872 - 2878. [Abstract] [Full Text] [PDF]

				L. Bello, V. Lucini, G. Carrabba, C. Giussani, M. Machluf, M. Pluderi, D. Nikas, J. Zhang, G. Tomei, R. M. Villani, et al. Simultaneous Inhibition of Glioma Angiogenesis, Cell Proliferation, and Invasion by a Naturally Occurring Fragment of Human Metalloproteinase-2 Cancer Res., December 1, 2001; 61(24): 8730 - 8736. [Abstract] [Full Text] [PDF]

				S. PFEIFFER, A. LASS, K. SCHMIDT, and B. MAYER Protein tyrosine nitration in mouse peritoneal macrophages activated in vitro and in vivo: evidence against an essential role of peroxynitrite FASEB J, November 1, 2001; 15(13): 2355 - 2364. [Abstract] [Full Text] [PDF]

				E. Noiri, A. Nakao, K. Uchida, H. Tsukahara, M. Ohno, T. Fujita, S. Brodsky, and M. S. Goligorsky Oxidative and nitrosative stress in acute renal ischemia Am J Physiol Renal Physiol, November 1, 2001; 281(5): F948 - F957. [Abstract] [Full Text] [PDF]

				L.-Y. Xu, J.-S. Yang, H. Link, and B.-G. Xiao SIN-1, a Nitric Oxide Donor, Ameliorates Experimental Allergic Encephalomyelitis in Lewis Rats in the Incipient Phase: The Importance of the Time Window J. Immunol., May 1, 2001; 166(9): 5810 - 5816. [Abstract] [Full Text] [PDF]

				B. Brooks, D. M. Briggs, N. C. Eastmond, D. G. Fernig, and J. W. Coleman Presentation of IFN-{gamma} to Nitric Oxide-Producing Cells: A Novel Function for Mast Cells J. Immunol., January 15, 2000; 164(2): 573 - 579. [Abstract] [Full Text] [PDF]

				A. Allione, P. Bernabei, M. Bosticardo, S. Ariotti, G. Forni, and F. Novelli Nitric Oxide Suppresses Human T Lymphocyte Proliferation Through IFN-{gamma}-Dependent and IFN-{gamma}-Independent Induction of Apoptosis J. Immunol., October 15, 1999; 163(8): 4182 - 4191. [Abstract] [Full Text] [PDF]

				M. Dlaska and G. Weiss Central Role of Transcription Factor NF-IL6 for Cytokine and Iron-Mediated Regulation of Murine Inducible Nitric Oxide Synthase Expression J. Immunol., May 15, 1999; 162(10): 6171 - 6177. [Abstract] [Full Text] [PDF]

				K. Funatogawa, M. Matsuura, M. Nakano, M. Kiso, and A. Hasegawa Relationship of Structure and Biological Activity of Monosaccharide Lipid A Analogues to Induction of Nitric Oxide Production by Murine Macrophage RAW264.7 Cells Infect. Immun., December 1, 1998; 66(12): 5792 - 5798. [Abstract] [Full Text] [PDF]

				J. J. Gao, M. B. Filla, M. J. Fultz, S. N. Vogel, S. W. Russell, and W. J. Murphy Autocrine/Paracrine IFN-{alpha}{beta} Mediates the Lipopolysaccharide-Induced Activation of Transcription Factor Stat1{alpha} in Mouse Macrophages: Pivotal Role of Stat1{alpha} in Induction of the Inducible Nitric Oxide Synthase Gene J. Immunol., November 1, 1998; 161(9): 4803 - 4810. [Abstract] [Full Text] [PDF]

				W. Dong, M. K. Selgrade, M. Ian Gilmour, R. W. Lange, P. Park, M. I. Luster, and F. W. Kari Altered Alveolar Macrophage Function in Calorie-restricted Rats Am. J. Respir. Cell Mol. Biol., September 1, 1998; 19(3): 462 - 469. [Abstract] [Full Text]

				A. K. Kiemer and A. M. Vollmar Autocrine Regulation of Inducible Nitric-oxide Synthase in Macrophages by Atrial Natriuretic Peptide J. Biol. Chem., May 29, 1998; 273(22): 13444 - 13451. [Abstract] [Full Text] [PDF]

				S. Recalcati, R. Pometta, S. Levi, D. Conte, and G. Cairo Response of Monocyte Iron Regulatory Protein Activity to Inflammation: Abnormal Behavior in Genetic Hemochromatosis Blood, April 1, 1998; 91(7): 2565 - 2572. [Abstract] [Full Text] [PDF]

				L. Ramamoorthy and I. R. Tizard Induction of Apoptosis in a Macrophage Cell Line RAW 264.7 By Acemannan, a beta -(1,4)-Acetylated Mannan Mol. Pharmacol., March 1, 1998; 53(3): 415 - 421. [Abstract] [Full Text]

				J. C. Zhuang and G. N. Wogan Growth and viability of macrophages continuously stimulated to produce nitric oxide PNAS, October 28, 1997; 94(22): 11875 - 11880. [Abstract] [Full Text] [PDF]

				M.J. Cody, C.A. Salkowski, B.E. Henricson, G.R. Detore, R.S. Munford, and S.N. Vogel Effect of inflammatory and antiinflammatory stimuli on acyloxyacyl hydrolase gene expression and enzymatic activity in murine macrophages Innate Immunity, October 1, 1997; 4(5): 371 - 379. [Abstract] [PDF]

				L. S. Feder, D. Stelts, R. W. Chapman, D. Manfra, Y. Crawley, H. Jones, M. Minnicozzi, X. Fernandez, T. Paster, R. W. Egan, et al. Role of Nitric Oxide on Eosinophilic Lung Inflammation in Allergic Mice Am. J. Respir. Cell Mol. Biol., October 1, 1997; 17(4): 436 - 442. [Abstract] [Full Text]