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Effect of Human Recombinant Granulocyte/Macrophage Colony-stimulating Factor and Native Granulocyte Colony-stimulating Factor on Clonogenic Leukemic Blast Cells¹

The First Department of Internal Medicine [Y. A., T. S., Y. N.] and Cancer Center [S. O., S. Y.], Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan, and DNAX Research Institute of Molecular and Cellular Biology [T. O.], Palo Alto, California 94304

The effects of human recombinant granulocyte/macrophage colony-stimulating factor (GM-CSF) and human native purified granulocyte colony-stimulating factor (G-CSF) on the growth of clonogenic leukemic blast cells from eight Japanese patients with acute myeloblastic leukemia were studied, using an in vitro leukemic blast colony assay. The results showed that GM-CSF stimulated leukemic blast colony formation in all cases examined, whereas G-CSF stimulated colony formation in four of the eight cases. The maximum stimulating activity of GM-CSF on the growth of clonogenic leukemic blast cells was higher than that of G-CSF in the majority of cases, while sometimes GM-CSF and G-CSF worked synergistically. Thus, the clonogenic leukemic blast cell populations seemed to be heterogeneous with respect to their in vitro response to growth regulators.

¹ This work was supported in part by Grants-in-Aid from the Ministry of Education, Science, and Culture, Japan (Nos. 61015074 and 61440054) and by the Fukuoka Anti-Cancer Society.

² To whom requests for reprints should be addressed.

Received 5/ 1/87. Revised 7/17/87. Accepted 7/28/87.