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Chemoprevention of Colon Carcinogenesis by Dietary Organoselenium, Benzylselenocyanate, in F344 Rats¹

Divisions of Nutrition and Endocrinology [B. S. R., P. M.], Experimental Pathology and Toxicology [H. M., S. S.], and Chemical Carcinogenesis [K. E.], Naylor Dana Institute for Disease Prevention, American Health Foundation, Valhalla, New York 10595

The effect of feeding benzylselenocyanate (BSC) and its sulfur analogue, benzylthiocyanate (BTC), 2 wk before, during, and until 1 wk after carcinogen administration (initiation phase) on intestinal carcinogenesis induced by azoxymethane (CAS:25843-45-2) was studied in male F344 rats. Weanling rats were raised on a semipurified diet (AIN-76A diet; control diet). Beginning at 5 wk of age, groups of animals consuming the control diet were fed one of the diets containing 25 ppm BSC or BTC. An additional group was continued on the control diet. At 7 wk of age, all animals in 3 groups, except the vehicle-treated controls, were administered s.c. injections of azoxymethane (15 mg/kg body weight, once weekly for 2 wk). Animals were continued on the control diet and BSC and BTC diets until 1 wk after carcinogen treatment, when those groups receiving BSC and BTC diets were fed the control diet until termination of the experiment. Tissue and blood plasma glutathione peroxidase activity was measured in vehicle-treated animals fed the control diet and BSC and BTC diets for 5 wk. The results indicate that body weights were comparable among the various dietary groups. BSC in the diet significantly inhibited the incidence (percentage of animals with tumors) and multiplicity (tumors/animal) of adenocarcinomas in the colon and multiplicity of adenocarcinomas in the small intestine compared to those fed the control diet. BTC in the diet had no effect on colon and small intestinal tumors. Selenium-dependent glutathione peroxidase activity was significantly increased in kidneys and colon and small intestinal mucosae of animals fed the BSC diet compared to animals fed the BTC and control diets.

¹ This investigation was supported by USPHS Grants CA17613 and CA36892 from the National Cancer Institute. Animals were maintained under the guidelines set forth in the "Guide for Care and Use of Laboratory Animal Resources" by the National Research Council. This is Paper 4 in the series, "Selenium in Chemoprevention of Carcinogenesis."

² To whom requests for reprints should be addressed.

Received 6/ 8/87. Revised 8/17/87. Accepted 8/21/87.

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