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Modulation of Human Hemopoietic Progenitor Cell Growth in Vitro by Recombinant Human ß-Interferon¹

Department of Medicine, New York Medical College, Valhalla, New York 10595

Interferons are known to have modulatory effects on hemopoiesis. Human bone marrow mononuclear cells were employed to test the effects of human recombinant ß-interferon on myeloid and erythroid hemopoietic stem cell growth. Results demonstrated that 1,000 U/ml of ß-interferon significantly inhibited myeloid growth [colony-forming unit (CFU)-granulocyte macrophage] by 40–50%, whereas a higher concentration (10,000 U/ml) abolished CFU-granulocyte macrophage growth by 80–100%. The inhibitory effects of ß-interferon were partially reversible by increasing the concentrations of colony stimulating activity in the culture and could not be abrogated by addition of toxic oxygen radical scavengers such as superoxide dismutase and catalase. The inhibitory effect of interferon was found to be partially dependent on the presence of accessory cells, since less inhibition was seen using T-cell and monocyte depleted bone marrow cells. Lower concentrations of ß-interferon (10–100 units/ml) were without effect. In contrast to myeloid cells, the human erythroid progenitors (CFU-erythroid, burst-forming unit-erythroid) appear to be more sensitive to the inhibitory effects of ß-interferon. In this regard it was found that 100 U/ml of ß-interferon suppressed erythroid growth by 40–50%. These results suggest that human recombinant ß-interferon is capable of suppressing hemopoietic colony growth.

¹ Presented in part at The Annual Meeting of the American Association for Cancer Research, May 1986 (27).

² Present address: Beijing Institute for Cancer Research, Beijing, People's Republic of China.

³ To whom requests for reprints should be at addressed.

Received 2/ 3/87. Revised 8/27/87. Accepted 9/21/87.