This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nara, P. L. Articles by Fischinger, P. J. Search for Related Content PubMed PubMed Citation Articles by Nara, P. L. Articles by Fischinger, P. J.

Lipoprotein-associated Oncornavirus-inactivating Factor in the Genus Mus: Effects on Murine Leukemia Viruses of Laboratory and Exotic Mice

Virus Control Unit, Office of the Director, NCI-Frederick Cancer Research Facility, Frederick, Maryland 21701 [P. L. N., N. M. D., W. G. R., P. J. F.], and Laboratory of Tumor Immunobiology and Biology, National Cancer Institute, Bethesda, Maryland 20892 [R. C.]

High titers of oncornavirus-inactivating factor (OIF) were found previously in sera of laboratory mice. OIF is highly active against mouse xenotropic and polytropic envelope recombinant murine leukemia viruses (MuLVs) but not against ecotropic MuLVs. Of the 20 different mouse species or subspecies currently tested, that represent 4 subgenera, no OIF was found in the 3 subgenera more distant to the laboratory mouse. In the subgenus Mus, 7 of the 8 most distant species had no OIF, whereas all the ancestral species and subspecies of the laboratory mouse (Mus musculus musculus, Mus musculus domesticus), including a more distant member (Mus musculus cookii), had ample titers of OIF. A new separation technique was devised so that potential virus-neutralizing immunoglobulins could be separated by electrophoresis from OIF in smallvolume serum samples. Active OIF was recovered from serum highdensity lipoprotein, from very-low-density lipoprotein, as well as from chylomicron fractions. Murine sarcoma virus pseudotypes were made with several available exotic MuLV types. These pseudotype MuLVs were not susceptible to standard OIF preparations. The sera of exotic mice also had no factor analogous to OIF which would inactivate their own homologous or heterologous exotic MuLVs. It appears that, with one exception, OIF activity is limited to two subspecies of M. musculus and may be correlated in these subspecies with the presence of endogenous xenotropic MuLVs.

¹ To whom requests for reprints should be addressed.

Received 9/ 5/86. Accepted 10/23/86.

This article has been cited by other articles:

				T. Wu, C. G. Lee, A. Buckler-White, and C. A. Kozak Genetic Control of a Mouse Serum Lipoprotein Factor That Inactivates Murine Leukemia Viruses: Evaluation of Apolipoprotein F as a Candidate J. Virol., March 1, 2002; 76(5): 2279 - 2286. [Abstract] [Full Text] [PDF]