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Biological Studies on the Main Fractions of Hematoporphyrin Derivative¹

Beckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, California 92717

The four main fractions of hematoporphyrin derivative were separated by high-pressure liquid chromatography. Each fraction was studied with respect to photosensitizing capabilities, fluorescence, and tumor tissue uptake in mice bearing EMT-6 tumors.

Animals received i.p. injections of 10 mg/kg of each fraction, and 24 h later tumors either were treated with 100 J/cm² of light (630 nm) to evaluate photosensitizing capabilities, or the animals were sacrificed and tumors removed for fluorescence and fraction uptake determination.

The results indicate that the fraction responsible for photosensitization has the highest tumor tissue uptake and retention. Furthermore, this fraction demonstrates the highest overall fluorescence localization in neoplastic tissue. The other poorly photosensitizing fractions have a lower overall fluorescence in vivo due to their poor tumor tissue localization.

¹ This research was supported by NIH Grants RR 01192 and CA 32248.

Received 8/25/86. Revised 11/ 4/86. Accepted 11/ 6/86.