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Identification and Partial Characterization of a M_r 40,000 Nucleolar Antigen Associated with Cell Proliferation¹

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030

The present study reports the identification and partial characterization of a novel M_r 40,000 nucleolar antigen (P40) by monoclonal antibodies. Monoclonal antibodies to this protein were obtained when a nucleolar protein extract separated from the immunodominant protein C23 was used to immunize BALB/c mice; 12 hybridoma clones produced antibodies to this protein. P40 was not detected in normal human kidney, liver, and leukocytes but was readily demonstrable in a variety of human malignant tissues. This newly identified P40 antigen differs in its specific nucleolar localization from cyclin (proliferating cell nuclear antigen), a M_r 35,000 antigen which is largely in the nucleoplasm. In addition, cyclin appears in the nucleolus in S-phase; P40 appears in the nucleolus 6 h after refeeding serum-starved HeLa cells.

¹ These studies were supported by Cancer Research Center Grant CA-10893, P1, awarded by National Cancer Institute, Department of Health and Human Services, Public Health Service; The DeBakey Medical Foundation; The Davidson Fund; The Pauline Sterne Wolff Memorial Foundation; H. Leland Kaplan Cancer Research Endowment; Linda & Ronny Finger Cancer Research Endowment Fund; and The William S. Farish Fund.

² To whom requests for reprints should be addressed, at Baylor College of Medicine, Department of Pharmacology, Texas Medical Center, One Baylor Plaza, Houston, TX 77030.

Received 8/25/86. Revised 11/14/86. Accepted 11/17/86.

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