This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shitara, K. Articles by Yoshida, H. Search for Related Content PubMed PubMed Citation Articles by Shitara, K. Articles by Yoshida, H.

Distribution of Lung Adenocarcinoma-associated Antigens in Human Tissues and Sera Defined by Monoclonal Antibodies KM-52 and KM-93

Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6, Asahi-machi, Machida-shi, Tokyo 194, Japan

Two monoclonal antibodies to human lung adenocarcinoma, KM-52 and KM-93, were generated by the novel immunizing procedure using mice rendered tolerant to the normal human lung (N. Hanai et al., Cancer Res., 46: 4438–4443, 1986). KM-93 recognized sialylated carbohydrate epitope on the antigen different from CA19-9 and DU-PAN-2, while KM-52 recognized the protein antigen. Both antigens were different from carcinoembryonic antigen, -fetoprotein, and ß₂-microglobulin.

Distribution of KA-52 and KA-93, the antigens recognized by KM-52 and KM-93, respectively, in various tissues and sera was investigated.

In immunoperoxidase staining, KM-93 reacted strongly and frequently with tumor cells of lung adenocarcinoma and partially with those of lung squamous cell carcinoma, large cell carcinoma, and small cell carcinoma. In normal adult and fetal tissues, KA-93 was expressed on the surface of a small number of cells of the lung, pancreas, liver, kidney, and bone marrow. KM-52 reacted selectively with tumor cells of adenocarcinoma among four different histological types of lung carcinoma. In normal adult and fetal tissues, KA-52 was distributed on a small number of cells of the lung, stomach, intestine, and pancreas.

Of the two monoclonal antibodies, KM-93 could be used in detecting the antigen in sera of patients with lung cancer. The KA-93 level in sera was determined by the sandwich-type enzyme-linked immunosorbent assay. Serum with a high KA-93 level was found in 34 of 70 patients with lung adenocarcinoma (48.6%), one of 67 healthy adults (1.5%), and none of 32 patients with benign diseases (0%). Combined detection by KA-93 with KA-32, a new tumor marker of lung squamous cell carcinoma (N. Hanai et al., Cancer Res., 46:5206–5210, 1986), elevated the positive percentage in patients with lung squamous cell carcinoma (52.7%) and with lung adenocarcinoma (59.5%).

These results suggested that KM-52 and KM-93 would be potential monoclonal antibodies in immunohistological diagnosis and serum diagnosis of lung adenocarcinoma, respectively.

¹ To whom requests for reprints should be addressed.

Received 3/21/86. Revised 8/ 1/86. Revised 11/24/86. Accepted 12/ 2/86.

This article has been cited by other articles:

				Y. Zhang, T. Ohkuri, D. Wakita, Y. Narita, K. Chamoto, H. Kitamura, and T. Nishimura Sialyl lewisx antigen-expressing human CD4+ T and CD8+ T cells as initial immune responders in memory phenotype subsets J. Leukoc. Biol., September 1, 2008; 84(3): 730 - 735. [Abstract] [Full Text] [PDF]

				Y. I. Kawamura, R. Kawashima, R. Fukunaga, K. Hirai, N. Toyama-Sorimachi, M. Tokuhara, T. Shimizu, and T. Dohi Introduction of Sda Carbohydrate Antigen in Gastrointestinal Cancer Cells Eliminates Selectin Ligands and Inhibits Metastasis Cancer Res., July 15, 2005; 65(14): 6220 - 6227. [Abstract] [Full Text] [PDF]

				A. Helander, K. J. Silvey, N. J. Mantis, A. B. Hutchings, K. Chandran, W. T. Lucas, M. L. Nibert, and M. R. Neutra The Viral {sigma}1 Protein and Glycoconjugates Containing {alpha}2-3-Linked Sialic Acid Are Involved in Type 1 Reovirus Adherence to M Cell Apical Surfaces J. Virol., July 15, 2003; 77(14): 7964 - 7977. [Abstract] [Full Text] [PDF]

				A. Sawano, S. Iwai, Y. Sakurai, M. Ito, K. Shitara, T. Nakahata, and M. Shibuya Flt-1, vascular endothelial growth factor receptor 1, is a novel cell surface marker for the lineage of monocyte-macrophages in humans Blood, February 1, 2001; 97(3): 785 - 791. [Abstract] [Full Text] [PDF]

				K. Nagata, T. Tsuji, K. Matsushima, N. Hanai, and T. Irimura Redistribution of selectin counter-ligands induced by cytokines Int. Immunol., April 1, 2000; 12(4): 487 - 492. [Abstract] [Full Text] [PDF]

				P. J. Giannasca, K. T. Giannasca, A. M. Leichtner, and M. R. Neutra Human Intestinal M Cells Display the Sialyl Lewis A Antigen Infect. Immun., February 1, 1999; 67(2): 946 - 953. [Abstract] [Full Text] [PDF]

				T. Gallart, D. Roelcke, M. Blay, A. Pereira, A. Martinez, O. Masso, O. Vinas, M. Cid, J. Esparza, R. Molina, et al. Anti-Sia-lb (Anti-Gd) Cold Agglutinins Bind the Domain NeuNAcalpha 2-3Gal in Sialyl Lewisx, Sialyl Lewisa, and Related Carbohydrates on Nucleated Cells and in Soluble Cancer-Associated Mucins Blood, August 15, 1997; 90(4): 1576 - 1587. [Abstract] [Full Text] [PDF]