This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kiss, Z. Articles by Kuo, J. F. Search for Related Content PubMed PubMed Citation Articles by Kiss, Z. Articles by Kuo, J. F.

Differential Effects of Various Protein Kinase C Activators on Protein Phosphorylation in Human Acute Myeloblastic Leukemia Cell Line KG-1 and Its Phorbol Ester-resistant Subline KG-1a¹

Departments of Pharmacology [Z. K., E. D., J. F. K.] and Medicine (Hematology and Oncology) [M. S., W. R. V.], Emory University School of Medicine, Atlanta, Georgia 30322; Department of Medicine (Hematology/Oncology) [H. P. K.], University of California at Los Angeles, Los Angeles, California 90024; and Cancer Research Institute and Department of Chemistry [G. R. P.], Arizona State University, Tempe, Arizona 85287

Human myeloid leukemia KG-1 cells are induced to differentiate to macrophage-like cells by tumor-promoting phorbol esters, such as 12-O-tetradecanoylphorbol-13-acetate (TPA). Cells from the cloned subline, KG-1a, unlike the parental line, are resistant to the differentiating effect of TPA. In the present studies, we investigated in these cells protein phosphorylation stimulated by various protein kinase C activators, including 1-oleoyl-2-acetylglycerol in the presence of the diacylglycerol kinase inhibitor R59022, TPA, mezerein, and bryostatin. All the agents stimulated, to a greater extent and with a higher potency, phosphorylation of several proteins in KG-1 cells than in KG-1a cells. On the other hand, these agents markedly stimulated phosphorylation of other proteins in KG-1a cells compared to that in KG-1 cells. The findings indicated that the actions of the diacylglycerol, 1-oleoyl-2-acetylglycerol, and the non-metabolizable activators (TPA, mezerein, and bryostatin) were very similar but not fully equivalent; and that KG-1a cells exhibited altered (increased or decreased) phosphorylation patterns, perhaps related to the TPA resistance characteristic of this subline of cells.

¹ Supported by USPHS Research Grants CA-36777, HL-15696, and NS-17608 (J. F. K.), CA-26038 and CA-32737 (H. P. K.), CA-29850 (W. R. V.), and CA-16094 and N01-CM-97262 (G. R. P.).

² Recipient of USPHS Research Career Development Award and a member of Johnson Cancer Center.

Received 7/30/86. Revised 11/13/86. Accepted 12/ 1/86.

This article has been cited by other articles:

				D. C. St. Louis, J. B. Woodcock, G. Fransozo, P. J. Blair, L. M. Carlson, M. Murillo, M. R. Wells, A. J. Williams, D. S. Smoot, S. Kaushal, et al. Evidence for Distinct Intracellular Signaling Pathways in CD34+ Progenitor to Dendritic Cell Differentiation from a Human Cell Line Model J. Immunol., March 15, 1999; 162(6): 3237 - 3248. [Abstract] [Full Text] [PDF]