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HL-60-1E3, a Novel Phorbol Diester-resistant HL-60 Cell Line¹

Division of Hematology/Oncology, Department of Medicine [J.A.L., B.A., R.E.H.], and Department of Microbiology/Immunology [J.A.L., R.E.H.], Medical College of Virginia, Virginia Commonwealth University, and Hunter Holmes McGuire VA Medical Center [P.C., B.A.], Richmond, Virginia 23298

The HL-60 (human promyelocytic leukemia) cell line has proved to be a useful model for the study of the expression of cellular functions and markers associated with hematopoietic differentiation. We report here the development and initial characterization of a novel, differentiation-resistant HL-60 cell line (HL-60-1E3) which was established by cloning the parent HL-60 line in the absence of mutagens or differentiation-inducing agents. HL-60-1E3 exhibits markedly reduced phorbol diester-induced expression of extracellular cytolytic activity, nonspecific esterase, phagocytosis, and surface Mo1 antigen. In addition, dimethyl sulfoxide-induced expression of both Mo1 and Mo2 is markedly reduced. However, if HL-60-1E3 is exposed to dimethyl sulfoxide, it acquires appreciable phorbol diester-triggered cytolytic activity and production of superoxide anion (O₂^-). Phorbol diester receptor number and dissociation constant (K_d) obtained by Scatchard analysis are not significantly different for the two cell lines. The HL-60-1E3 cell line should provide a useful adjunct to other cell lines used in the study of normal myeloid and leukemic cell differentiation, as well as the study of cytokine maturation factors and oncogene expression.

¹ Supported by NIH Grant CA37026 and the Thomas F. Jeffress and Kate Miller Jeffress Memorial Trust.

² To whom requests for reprints should be addressed, at Department of Medicine, Box 230 MCV Station, Medical College of Virginia, Richmond, VA 23298.

³ Recipient of National Heart, Lung and Blood Institute Clinical Investigator Award HL01053.

⁴ John A. and George L. Hartford Fellow.

Received 7/28/86. Revised 11/13/86. Accepted 11/14/86.