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Enhanced Immunogenicity of the Cultured Rat Fibrosarcoma KMT-17 by Cultivation in a Low Concentration of Fetal Calf Serum¹

Laboratory of Pathology [I. C., M. T., K. Y., H. K.] and Laboratory of Molecular Genetics [T. O., S. Y.], Cancer Institute, Hokkaido University School of Medicine; Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University [M. N., I. M.]; and Central Clinical Laboratory, Hokkaido University Dental Hospital [S. M.], Sapporo 060, Japan

We examined in different culture conditions alterations in the tumorigenicity and immunogenicity of an A3 clone that had been derived from a rat fibrosarcoma KMT-17. When a fetal calf serum concentration in a culture medium was lowered from 10 to 1%, the tumorigenicity was diminished while the immunogenicity was enhanced in a reversible manner; this was accompanied by a reversible prolongation of the in vitro doubling time. These phenomena were not due to an increase in the quantities of the original tumor-associated antigen and/or of the rat major histocompatibility complex (RT1) but seemed to be due to the appearance of a unique antigen(s) that was detected by an antibody taken from rats immunized with A3 tumor cells cultured in the low fetal calf serum concentration; this antigen(s) may consist of glycoprotein and exist as a crypt antigen(s). These phenomena were measured by an absorption test and flow cytometric analysis. Our observations suggest that the in vitro culture condition of tumor cells, in particular their culturing in the low fetal calf serum concentration medium, modifies the surface of tumor cells and causes a diminishment in their tumorigenicity and an enhancement of their immunogenicity.

¹ This work was supported by a Grant-in-Aid for Cancer Research from the Japanese Ministry of Education, Science, and Culture.

² To whom requests for reprints should be addressed.

Received 7/31/86. Revised 12/ 1/86. Accepted 12/23/86.