This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tolsma, S. S. Articles by Bouck, N. Search for Related Content PubMed PubMed Citation Articles by Tolsma, S. S. Articles by Bouck, N.

Genetic Assessment of the Strength of a Cancer Suppressor Gene in Hamster Cells¹

Departments of Microbiology-Immunology and Pathology and Cancer Center, Northwestern University Medical and Dental Schools, Chicago, Illinois 60611

Tetraploid and near-tetraploid chemically transformed derivatives of the pseudodiploid hamster line BHK 21/clone 13 were prepared in four different ways and their ability to be suppressed for anchorage independence by fusion to the anchorage-dependent parental line was tested. In all cases the presence of a single normal genome, thought on genetic grounds to contain a single suppressor gene, was able to prevent the anchorage-independent growth of transformed lines whether they contained one or two complements of pseudodiploid chromosomes. Suppression of the single step in carcinogenesis that is registered by BHK cells as they transform to anchorage independence is thus unusually powerful and apparently independent of chromosome balance and of strict dosage effects.

¹ Supported by NIH Grant CA 27306. The Northwestern University Flow Cytometry Program is supported by a generous gift from the Coleman Foundation.

² To whom reprint requests should be addressed, at 303 East Chicago Avenue, Chicago, IL 60611.

Received 6/24/87. Revised 9/23/87. Accepted 9/29/87.