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Fibrin-Fibronectin Compounds in Human Ovarian Tumor Ascites and Their Possible Relation to the Tumor Stroma¹

Frauenklinik [O. W., R. H., M. P., M. S., H. G.] und Institut für Allgemeine Pathologie und Pathologische Anatomie der Technischen Universität [E. C., R. B., W. G.], München; Institut für Immunologie [R. L.] der Ludwig-Maximilians-Universität, Munich, Federal Republic of Germany

Covalently linked heterogeneous fibrin-fibronectin compounds were detected in ascitic fluid of 31 patients with advanced ovarian cystadenocarcinoma by means of enzyme-linked immunosorbent assay techniques, immunoaffinity chromatography, and Western blot analysis. Deposition of fibrin and fibronectin could also be demonstrated immunohistochemically in Carnoy-fixed tissue sections. Fibrin and fibronectin were found in the tumor stroma within tumor nests and more prominently in stroma surrounding the tumor nests. The association of fibrin and fibronectin was especially pronounced in the stroma surrounding the tumor islands. Fibronectin was also found to be associated with stroma cells. Areas within the tumor stroma showed superimposed staining for both fibrin and fibronectin supporting the assumption that the covalently linked fibrin-fibronectin conjugates found in ascitic fluid may stem from the provisional tumor stroma by proteolytic release.

¹ This work was supported by the Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg, FRG (Sonderforschungsbereich 207, A-2, Munich).

² To whom requests for reprints should be addressed, at Frauenklinik der Technischen Universität München, Ismaningerstrasse 22, D-8000 Munich, West Germany.

Received 8/18/87. Revised 2/29/88. Accepted 3/14/88.

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