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Changes in Expression of Oncogenes and Endogenous Retroviral-like Sequences during Colon Carcinogenesis¹

Departments of Surgery [J. G. G., K. A. F., P. L.], and Pathology [K. M. O.], Division of Environmental Sciences and Institute of Cancer Research [L. L. H., I. B. W.], Columbia University, New York, New York 10032

The possible roles in experimental colon carcinogenesis of two protooncogenes (c-myc and c-H-ras), two endogenous retrovirus-related DNA sequences [rat leukemia virus (RaLV) and the 30S sequence], and two cell cycle related genes (ß-actin and ornithine decarboxylase) were studied by analyzing the levels of their corresponding RNAs during the course of azoxymethane induced and high fat promoted colon carcinogenesis. F-344 male rats received three S.C. injections of azoxymethane (15 mg/kg) or normal saline and were then subdivided into high or low fat diet groups. During subsequent serial sacrifices normal colon mucosa, adenomas, and carcinomas were harvested for histology and RNA extraction. Seventy-one RNA samples were analyzed by the Northern blot hybridization procedure using the appropriate ³²P-labeled DNA probes. A marked increase in the abundance of c-myc, RaLV, and 30S RNAs were seen in all of the colon tumors, including adenomas and invasive carcinomas. No or a very low level of expression of RaLV and c-myc RNA was found in the flat grossly normal mucosa adjacent to the tumors and in the mucosa of the control rats. Some of the colon tumors also displayed increased levels of c-H-ras, ornithine decarboxylase and ß-actin RNAs but these findings were less striking and more variable than those seen with c-myc, RaLV, and 30S RNAs. These results suggest that increased expression of the c-myc protooncogene and of the endogenous retrovirus-like sequences (RaLV) and 30S are hallmarks of colon carcinogenesis in this model system.

¹ This work was supported by National Cancer Institute Grant CA 02111, an award from the National Foundation for Cancer Research (to I. B. W.), and the Jean and Louis Dreyfus Foundation.

² Recipient of a Phyllis Jacobs Rubenstein-Arthur Jacobs Fellowship in Surgical Oncology. To whom requests for reprints should be addressed.

Received 9/22/87. Revised 2/ 1/88. Accepted 3/31/88.

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