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Multiparametric Evaluation (SAMBA) of Growth Fraction (Monoclonal Ki67) in Breast Carcinoma Tissue Sections¹

From the Department of Pathology, Timone Hospital, Marseille, France

Breast tissue samples, including normal breast, nonmalignant disorders, and breast carcinomas (n = 257), were tested with monoclonal antibody Ki67 to define the growth fraction in each tissue subgroup. Immunocytochemical assays using anti-Ki67 and avidin-biotin-peroxidase complex and/or alkaline phosphatase anti-alkaline phosphatase were applied in frozen sections. The immunoreactions were analyzed with a computerized system of image analysis referred to as SAMBA (Systeme d'Analyse Microphotometrique à Balayage Automatique). This system permitted a multiparametric and automatized analysis of colored images. The results obtained were: (a) the SAMBA analysis of Ki67-positive staining was accurate, reliable, and reproducible; (b) the anti-Ki67 immunostaining was significantly (P < 0.01) increased in malignancies and was related to the tumors' degree of differentiation, the vascular invasion, and the presence of axillary lymph node metastases; (c) anti-Ki67 immunostaining is increased (P < 0.01) in tumors in which estrogen receptor and progesterone receptor antigenic sites are not detected. It is concluded that the SAMBA analysis of the anti-Ki67 immunocytochemical assay provides relevant information in selecting subgroups of patients with higher risk for relapse.

¹ This study was supported partly by grants from the LNLC, the ARC, the GEFLUC, the CR of Marseille and grant from Marseille Medical School.

² To whom requests for reprints should be addressed, at Laboratoire d'Anatomie Pathologique (Pr. M. Toga), Faculté de Médecine, 27 Bd Jean Moulin 13385, Marseille Cedex 05, France.

Received 12/11/87. Revised 3/29/88. Accepted 4/25/88.

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