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Profiles of Prostaglandin Biosynthesis in Sixteen Established Cell Lines Derived from Human Lung, Colon, Prostate, and Ovarian Tumors¹

Program Development Research Group [W. C. H., M. C. A., T. L. M.] and Developmental Therapeutics Program [M. R. B.], National Cancer Institute-Frederick Cancer Research Facility, Frederick, Maryland 21701-1013

The profiles of prostanoid biosynthesis from endogenous arachidonic acid in 16 established cell lines derived from 4 histological classes of human carcinomas were determined by capillary gas chromatographymass spectrometry. Detectable quantities of prostanoids were isolated from the culture medium of cell lines representative of the different histological classes of human tumors: colorectal adenocarcinomas (one of three cell lines); ovarian adenocarcinomas (one of three cell lines); prostate adenocarcinomas (zero of two cell lines); non-small cell carcinomas of the lung (four of five cell lines); and small cell carcinomas of the lung (zero of three cell lines). Prostaglandins E₂ and F₂ were the only prostanoids synthesized in detectable quantities. Prostaglandin E₂ biosynthesis (mean ± SD), pmol/10⁶ cells, n = 4) in cell lines exhibiting positive prostaglandin H synthase activity was: LoVo (colorectal adenocarcinoma, 0.4 ± 0.1); A2780 (ovarian adenocarcinoma, 1.3 ± 0.3); NCI-H322 (bronchioloalveolar cell carcinoma, 8.4 ± 3.1); NCI-H358 (bronchioloalveolar cell carcinoma, 7.8 ± 2.4); EKVX (adenocarcinoma of the lung, 21.3 ± 5.5); and A427 (large cell undifferentiated carcinoma of the lung, 12.6 ± 2.8). Prostaglandin F₂ production (pmol/10⁶ cells ± SD) was: LoVo (0.3 ± 0.1); NCI-H322 (0.6 ± 0.2); NCI-H358 (0.4 ± 0.1); EKVX (1.8 ± 0.4); and A427 (11.1 ± 3.1). These findings suggest that within certain limitations cultured tumor cells provide simplified experimental systems for determination of prostaglandin biosynthetic characteristics of human tumors and that prostanoid biosynthesis may be particularly characteristic of certain non-small cell carcinomas of the lung.

¹ This project has been funded at least in part with Federal funds from the Department of Health and Human Services under Contract N01-CO-74102. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the United States Government.

² To whom requests for reprints should be addressed, at the Program Development Research Group, Building 560, Room 32–60, Frederick, MD 21701-1013.

Received 2/23/88. Revised 5/25/88. Accepted 6/ 3/88.

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