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Analysis of Human Papillomavirus Sequences in Cell Lines Recently Derived from Cervical Cancers

Wellcome Research Laboratories, Langley Court, Beckenham, Kent, England BR3 3BS [R. P. S., L. B.]; Laboratory of Molecular Virology, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, WC2 3PX, England [A. M., L. C.]; and Radiotherapy Research Unit, Institute of Cancer Research, Clifton Avenue, Sutton, SM2 5PX, England [L. R. K.]

The DNA and RNA from four cell lines recently derived from cervical carcinomas (HX151c, HX155c, HX156c, and HX160c) were analyzed for the presence of human Papillomavirus DNA. Each contained HPV-16 DNA in a multicopy integrated form of varying complexity. Each also expressed RNA transcripts of similar sizes to CaSki cell transcripts. The splice acceptor position within the E6 coding region of the cell line HX156c was identical to CaSki, SiHa, and HeLa cells as well as a cell line derived from mouse fibroblasts transformed with HPV-16 sequences in a retrovirus vector. Immunoprecipitation with an anti-HPV-16 E6 polyclonal antiserum demonstrated that HX160c cells contain the E6 polypeptide derived from unspliced transcripts.

¹ To whom requests for reprints should be addressed.

Received 5/18/87. Revised 10/ 6/87. Accepted 10/13/87.