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Correlation between the Anticellular and DNA Fragmenting Activities of Tumor Necrosis Factor¹

Department of Lymphokine Biology, New York Blood Center, New York, New York 10021

The treatment of cells sensitive to the anticellular effect of tumor necrosis factor (TNF) with TNF results in a degradation of their cellular DNA into DNA fragments that are multiples of 200 base pairs. TNF treatment of cells resistant to the anticellular effect of TNF, but bearing receptors for TNF, fails to result in any DNA fragmentation. Incubation conditions, such as temperature, the presence of metabolic inhibitors or amino acid deprivation, that modulate the effectiveness of TNF or affect the rate at which TNF exerts its anticellular effect have a similar effect on the ability of the TNF to generate DNA fragments. Thus the TNF-mediated DNA fragmentation and the rate at which it occurs correlates with the rate at which cells respond to the anticellular effect of TNF and, as such, might serve as a marker for the responsiveness of cells to TNF.

¹ This work was supported by USPHS Grants CA-40614 and CA-38661 of the National Cancer Institute.

² To whom requests for reprints should be addressed, at the New York Blood Center, 310 East 67th Street, New York, NY 10021.

Received 6/ 6/88. Revised 8/ 3/88. Accepted 8/ 5/88.

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