This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Peyrat, J-P. Articles by Demaille, A. Search for Related Content PubMed PubMed Citation Articles by Peyrat, J-P. Articles by Demaille, A.

Insulin-like Growth Factor 1 Receptors in Human Breast Cancer and Their Relation to Estradiol and Progesterone Receptors¹

Centre Oscar Lambret, Rue F. Combemale, BP 307, 59020 Lille Cédex [J.-P. P., J. B., A. D.]; Centre d'Étude et de Recherche en Informatique Médicale, Faculté de Médecine, 59037 Lille [R. B.]; and Unité d'Endocrinologie Moléculaire INRA, 78350 Jouy-en-Josas [J. D.], France

Insulin-like growth factor 1 (IGF1) binding sites were characterized in breast cancer. We demonstrate the presence of one high affinity binding site. Chemical cross-linking of ¹²⁵I-IGF1 to breast cancer membranes in reducing condition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed one band with an apparent molecular weight of 130,000. The specificity of the binding was studied. IGF2 was a good competitor whereas insulin competed with a potency lower than 1/100 that of IGF1. This IGF1 binding corresponded to the previously described type 1 IGF receptor (IGF1-R). IGF1-R was determined in 76 human breast cancer biopsies. Ninety-three % of the tumors were positive. The specific binding range was 0–16.4%; the geometric IGF1-R mean level was 3.9%. There was a relation (x² test) between IGF1-R and progesterone receptor positivity rates (P = 0.002). The IGF1-R concentrations were correlated (Spearman test) with those of estradiol receptor (P = 0.0018) and progesterone receptor (P = 0.0011). A positive linear correlation existed between IGF1-R and estradiol receptor (P = 0.006) and between IGF1-R and progesterone receptor (P = 0.003). Our demonstration of the presence of IGF1-R in human breast cancer biopsies suggests that IGF1, acting either via the endocrine, paracrine, or autocrine pathways, could stimulate tumor growth.

¹ This work was supported by the Association pour la Recherche sur le Cancer (Villejuif) and by the Fédération Nationale des Centres de Lutte contre le Cancer.

Received 7/ 1/87. Revised 6/14/88. Accepted 8/10/88.

This article has been cited by other articles:

				F. Revillion, M. Charlier, V. Lhotellier, L. Hornez, S. Giard, M.-C. Baranzelli, J. Djiane, and J.-P. Peyrat Messenger RNA expression of leptin and leptin receptors and their prognostic value in 322 human primary breast cancers. Clin. Cancer Res., April 1, 2006; 12(7 Pt 1): 2088 - 2094. [Abstract] [Full Text] [PDF]

				F. Gizard, R. Robillard, O. Barbier, B. Quatannens, A. Faucompre, F. Revillion, J.-P. Peyrat, B. Staels, and D. W. Hum TReP-132 Controls Cell Proliferation by Regulating the Expression of the Cyclin-Dependent Kinase Inhibitors p21WAF1/Cip1 and p27Kip1 Mol. Cell. Biol., June 1, 2005; 25(11): 4335 - 4348. [Abstract] [Full Text] [PDF]

				A. Chotteau-Lelievre, F. Revillion, V. Lhotellier, L. Hornez, X. Desbiens, V. Cabaret, Y. de Launoit, and J.-P. Peyrat Prognostic Value of ERM Gene Expression in Human Primary Breast Cancers Clin. Cancer Res., November 1, 2004; 10(21): 7297 - 7303. [Abstract] [Full Text] [PDF]

				E Nardon, I Buda, G Stanta, E Buratti, M Fonda, and L Cattin Insulin-like growth factor system gene expression in women with type 2 diabetes and breast cancer J. Clin. Pathol., August 1, 2003; 56(8): 599 - 604. [Abstract] [Full Text] [PDF]

				B. A. Stoll Oestrogen/insulin-like growth factor-I receptor interaction in early breast cancer: clinical implications Ann. Onc., February 20, 2002; 13(2): 191 - 196. [Abstract] [Full Text] [PDF]

				V. Pawlowski, F. Revillion, M. Hebbar, L. Hornez, and J.-P. Peyrat Prognostic Value of the Type I Growth Factor Receptors in a Large Series of Human Primary Breast Cancers Quantified with a Real-Time Reverse Transcription-Polymerase Chain Reaction Assay Clin. Cancer Res., November 1, 2000; 6(11): 4217 - 4225. [Abstract] [Full Text] [PDF]

				H. Yu and T. Rohan Role of the Insulin-Like Growth Factor Family in Cancer Development and Progression J Natl Cancer Inst, September 20, 2000; 92(18): 1472 - 1489. [Abstract] [Full Text] [PDF]