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Isolation and Characterization of Colon Cancer Mucin from Xenografts of LS174T Cells¹

Gastrointestinal Research Laboratory, Veterans Administration Medical Center; and Department of Medicine, School of Medicine, University of California, San Francisco, California

The structure of colonic mucin, which is thought to be important in several diseases, including ulcerative colitis and colon cancer, is poorly understood. Mucin was isolated from nude mouse xenografts of the LS174T colonic adenocarcinoma cell line by gel filtration and CsCl density gradient centrifugation. The isolated mucin had a high content of threonine, serine, and proline, with 28% of the total amino acids O-glycosylated. The carbohydrates present were fucose, sialic acid, galactose, N-acetyl-glucosamine, and N-acetyl-galactosamine in the ratio of 0.4:1.5:1.0:0.9:1.4. Rabbit antibodies were prepared that recognized primarily protein-dependent determinants. By DEAE-cellulose chromatography, the purified mucin was found to be heterogeneous, with three major components that had small differences in carbohydrate composition. LS174T was antigenically and chromatographically similar to mucins in colon cancer tissue specimens and in nonmalignant colonic mucosae.

¹ This work was supported by the Veterans Administration Medical Research Service.

² To whom reprint requests should be addressed.

³ Charge de Recherche of INSERM. Present address Laboratoire d'Immunochimie, Institut de Recherches Scientifiques sur le Cancer, 94802 Villejuif, France.

⁴ Medical Investigator of the Veterans Administration.

Received 1/25/88. Revised 6/ 7/88. Accepted 8/26/88.

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