This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hiraiwa, N. Articles by Imura, H. Search for Related Content PubMed PubMed Citation Articles by Hiraiwa, N. Articles by Imura, H.

Monoclonal Antibodies Directed to a Disulfated Glycosphingolipid, S_B1a (GgOse₄Cer-II³IV³-bis-sulfate), Associated with Human Hepatocellular Carcinoma¹

Department of Clinical Science and Laboratory Medicine [N. H., S. I., K. S., R. K.], and The Second Division of the Department of Internal Medicine [N. H., Y. F., H. I.], Kyoto University, School of Medicine, Shogoin Kawaharacho 54, Sakyo-ku, Kyoto 606, and The Second Division of the Department of Biochemistry, Teikyo University, School of Medicine, Tokyo 173 [N. I., I. I.], Japan

Two murine monoclonal antibodies, 2H6G5 (IgM) and 4A9E10 (IgG₃), were obtained by using either cultured human hepatocellular carcinoma cells (PLC/PRF/5) or the acidic glycolipid mixture prepared from the same cells as immunogens. The antigen in PLC/PRF/5 cell membranes recognized by both antibodies was identified as a disulfated acidic glycolipid, GgOse₄Cer-II³IV³-bis-sulfate (S_B1a). Both antibodies reacted specifically with S_B1a, and no significant reactivity was noted with other sulfated glycolipids or gangliosides except that the antibody 2H6G5 showed a weak cross-reactivity with LacCer-II³-sulfate (S_M3), another sulfated glycolipid which partly shares the same carbohydrate structure as S_B1a. The S_B1a antigen is a relatively minor glycolipid in PLC/PRF/5 cells, but it was strongly expressed at the surface of PLC/PRF/5 cells as ascertained by cytofluorometry using both antibodies. A significant amount of S_B1a antigen was present in 3 of the acidic glycolipid fractions isolated from 15 human hepatocellular carcinoma tissues as well as in the acidic glycolipid fraction prepared from PLC/PRF/5 cells, while all the acidic glycolipid fractions prepared from cirrhotic livers and a normal liver were essentially negative for S_B1a, as ascertained by both solid phase enzyme immunoassay and the thin-layer chromatography-immunostaining method. These results strongly suggest that the S_B1a antigen as defined by these new monoclonal antibodies is associated with human hepatocellular carcinoma.

¹ This work was supported in part by a grant-in-aid for Cancer Research from the Ministry of Education, Science and Culture, Japan, and grants-in-aid from the Japanese Foundation for Multidisciplinary Treatment of Cancer, the Uehara Memorial Foundation, and the Princess Takamatsu Cancer Research Foundation, to R. K.

² To whom requests for reprints should be addressed.

Received 4/19/88. Revised 8/ 5/88. Accepted 8/11/88.

This article has been cited by other articles:

				M. Kyogashima, K. Tamiya-Koizumi, T. Ehara, G. Li, R. Hu, A. Hara, T. Aoyama, and R. Kannagi Rapid demonstration of diversity of sulfatide molecular species from biological materials by MALDI-TOF MS Glycobiology, August 1, 2006; 16(8): 719 - 728. [Abstract] [Full Text] [PDF]

				H. Ideo, A. Seko, and K. Yamashita Galectin-4 Binds to Sulfated Glycosphingolipids and Carcinoembryonic Antigen in Patches on the Cell Surface of Human Colon Adenocarcinoma Cells J. Biol. Chem., February 11, 2005; 280(6): 4730 - 4737. [Abstract] [Full Text] [PDF]