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Differential Extraction of Tumor-specific Antigens from Two Ultraviolet Light-induced Murine Fibrosarcomas with the Use of 1-Butanol¹

Department of Immunology, The University of Texas M. D. Anderson Cancer Center, Houston, Texas 77030

The purpose of this study was to investigate the immunobiological characteristics of the tumor-specific cell surface antigen expressed by the UV-induced murine fibrosarcoma, UV-2240. UV-2240 is classified as a regressor UV tumor because it is immunologically rejected by normal syngeneic mice but grows in immunocompromised or UV-irradiated hosts. The strong tumor-specific rejection antigen expressed by UV-2240 was found on the plasma membrane, and unlike the previously characterized antigen of UV-1591, the UV-2240 antigen was removed by using the noncytolytic butanol extraction technique. The tumor antigen activity in butanol extracts was resistant to digestion by endoglycosidase F and -mannosidase, but was destroyed by pronase. In addition, the immunoprotective activity in extracts of UV-2240 was thermostable. These data demonstrate that the UV-2240-specific tumor antigen possesses physico-chemical properties distinct from those of its well-characterized counter-part UV-1591.

¹ Supported in part by the University of Texas Cancer Foundation, and Grants CA-38500 and RR5511 awarded by the National Cancer Institute, Department of Health and Human Services.

² Recipient of an R. E. "Bob" Smith Predoctoral Fellowship.

³ To whom requests for reprints should be addressed, at Department of Immunology, Box 178, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030.

Received 6/ 6/88. Accepted 9/13/88.