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Characterization of a Nuclear Antigen (M_r 150,000) Associated with Cell Proliferation¹

Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030

In the course of development of a "library" of monoclonal antibodies to nucleolar proteins, a monoclonal antibody to a nuclear antigen with a molecular weight of 150,000 was obtained. Using this monoclonal antibody as an immunocytochemical probe, low immunofluorescence was demonstrated in human peripheral blood lymphocytes or HL-60 cells treated with retinoic acid. In contrast, a high degree of immunofluorescence was detected in rapidly proliferating human cells (HeLa, Hep-2, HL-60), in Novikoff hepatoma cells, and in phytohemagglutinin-activated human blood lymphocytes. Immunodot blot analysis indicated there were low levels of M_r 150,000 protein in quiescent lymphocytes and increasing amounts after stimulation with phytohemagglutinin. The increased level of M_r 150,000 protein thus is associated with cell proliferation.

¹ These studies were supported by Cancer Research Center Grant CA-10893, P1, awarded by the National Cancer Institute, Department of Health and Human Services, USPHS; the DeBakey Medical Foundation; the H. Leland Kaplan Cancer Research Endowment; the Linda and Ronnie Finger Cancer Research Endowment Fund; and the William S. Farish Fund.

² To whom requests for reprints should be addressed.

Received 5/ 4/88. Revised 8/ 5/88. Accepted 9/14/88.