This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Starling, J. J. Articles by Johnson, D. A. Search for Related Content PubMed Articles by Starling, J. J. Articles by Johnson, D. A.

Tissue Distribution and Cellular Location of the Antigens Recognized by Human Monoclonal Antibodies 16.88 and 28A32

Lilly Research Laboratories, Lilly Corporate Center, Indianapolis, Indiana 46285 [J. J. S., P. M., D. A. J.]; Department of Human Tumor Immunology and Pathology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021 [R. J. C.]; and Department of Pathology, Duke University, Durham, North Carolina 27710 [M. J. B.]

Results from a previous study (M. V. Haspel et al., Cancer Res., 45: 3951–3961, 1985) indicated that it was possible to isolate a high proportion of human monoclonal antibodies reactive with cell surface, tumorassociated antigens when the hybridomas were obtained from fusions utilizing peripheral blood lymphocytes from patients immunized with autologous tumor cells. The assignment of membrane reactivity was made from immunoperoxidase studies which used air-dried, nonpermeabilized Cytospin preparations of colon tumor cells. Tumor specificity was assessed by immunohistological assays by using frozen sections of normal and malignant human tissues. We now describe a series of studies using two of these antibodies, 16.88 and 28A32, in which further information was obtained concerning the tumor specificity and cellular location of the target antigens reactive with these monoclonal antibodies. Data were acquired from a variety of experimental techniques which included quantitative and qualitative immunofluorescence on live and permeabilized cells, RBC-rosetting assays, immunoperoxidase studies on Cytospin and frozen tissue sections, and immunoblot procedures. These studies show that the 16.88 and 28A32 human monoclonal antibodies bind to antigens which (a) are located in the cell cytoplasm and are not expressed in detectable levels on the cell surface, and (b) are found in many normal and malignant cell types.

¹ To whom requests for reprints should be addressed, at Lilly Research Laboratories, Department MC620, Building 98C/4, Lilly Corporate Center, Indianapolis, IN 46285.

Received 6/10/88. Revised 8/30/88. Accepted 9/ 8/88.