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Marker Polypeptides Distinguishing between Cancer Cell Clones with High and Low Potential for Spontaneous Metastasis in Murine Fibrosarcoma Cells¹

Institute of Pathology [I. A. G.] and Institute of Clinical Biochemistry [A. K. T., E. J.], University of Oslo, Rikshospitalet—The National Hospital, N-0027 Oslo I, Norway

Consistent differences in expression of specific proteins were observed between numerous cancer cell clones with high and low potential for spontaneous metastasis. Seventeen clones from two unrelated murine fibrosarcomas were examined concomitantly for spontaneous formation of lung metastases and for occurrence of individual polypeptide differences by high resolution two-dimensional gel electrophoresis. One of the identified marker polypeptides, designated Hi:2, was very strongly expressed by all 10 strongly metastatic clones, but was absent from 6 and only weakly expressed by one of the 7 weakly metastatic clones. Another marker polypeptide, designated Lo:6, was consistently most strongly expressed by the weakly metastatic clones. Among the approximately 2000 individually separated cellular polypeptides, only these two marker polypeptides consistently distinguished between the strongly and weakly metastatic clones from both fibrosarcomas. Five other polypeptides also distinguished between highly and weakly metastatic clones, but not as stringently.

¹ Grant support was received from the Norwegian Cancer Society, Grethe Harbitz' legat, Grosserer N. A. Stangs legat, and Nansenfondet og de dermed forbundne fond.

² Research Fellow of the Norwegian Cancer Society and of the Norwegian Research Council for Science and the Humanities. To whom requests for reprints should be addressed.

Received 6/ 8/87. Revised 10/ 8/87. Accepted 10/13/87.