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In Vivo ³¹P Nuclear Magnetic Resonance Spectroscopy of Subcutaneous 9L Gliosarcoma: Effects of Tumor Growth and Treatment with 1,3-Bis(2-chloroethyl)-1-nitrosourea on Tumor Bioenergetics and Histology¹

Departments of Radiology [R. G. S., K. A. M., S. S. R., J. P. W., J. D. G.], Neurological Surgery [R. J. T., H. B.], Oncology [H. B.], and Biological Chemistry [J. D. G.], The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

In vivo ³¹P nuclear magnetic resonance spectroscopy was used to examine the bioenergetics of the rat 9L gliosarcoma during untreated growth and in response to chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea. Tumor growth was associated with a decline in the phosphocreatine and nucleoside triphosphate resonances, consistent with an increase in tumor hypoxia during untreated growth. Following chemotherapy with 1,3-bis(2-chloroethyl)-1-nitrosourea (10 mg/kg), tumor levels of phosphocreatine and nucleoside triphosphate rebounded while the level of inorganic phosphate in the tumor declined. Histological comparison of treated and untreated tumor sections 4 days posttreatment showed that the treated tumor had a lower proportion of necrotic cells, a higher proportion of viable cells, and a 5-fold higher level of interstitial space than the control tumor.

¹ Supported in part by USPHS Grants CA 39958 and CA 44703 from The NIH (to J. D. G.), NIH Grant NS 01508-01 and Grant IN-11W from the American Cancer Society (to H. B.), NRSA Traineeship CA 09199 from the NIH (to R. G. S.), and by the Association for Brain Tumor Research (to R. J. T.).

² To whom requests for reprints should be addressed.

Received 8/10/87. Revised 10/26/87. Accepted 10/30/87.

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