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Spontaneous Sister Chromatid Exchange in Normal Bone Marrow and Ph-positive Chronic Myelocytic Leukemia¹

Universitätsklinikum Essen, Westdeutsches Tumorzentrum, Essen 1, Federal Republic of Germany

The frequency of spontaneous sister chromatid exchange was studied in normal marrow derived from 38 healthy donors and 40 untreated patients with chronic phase CML. The sister chromatid exchange frequency was significantly lower in the leukemic cells (range, 2.32 ± 1.31 to 4.76 ± 2.37 per metaphase; mean, 3.18 ± 0.49) than in normal marrow (range, 2.36 ± 1.44 to 5.54 ± 2.24 per metaphase; mean, 3.92 ± 0.72). The contraction status of chromosomes was comparable in normal and Ph-positive metaphases. The reduction of sister chromatid exchange in leukemic cells was seen in all chromosome groups. The analysis of cell cycle specific proliferation according to the typical staining patterns of metaphases due to the number of cell cycles during which bromodesoxyuridine was substituted, revealed longer cell cycle times for the leukemic cells.

¹ This study was supported by the Deutsche Forschungsgemeinschaft project A7, SFB 102.

² To whom requests for reprints should be addressed, at Innere Universitäts- und Poliklinik (Tumorforschung), Universitätsklinikum Essen, Westdeutsches Tumorzentrum, Hufelandstr. 55, D-4300 Essen 1, Federal Republic of Germany.

Received 5/12/87. Revised 10/ 6/87. Accepted 10/13/87.